546-0335/02 – Instrumental Methods of Analysis I (IMA I)
| Gurantor department | Department of Environmental Engineering | Credits | 8 |
| Subject guarantor | doc. Mgr. Eva Pertile, Ph.D. | Subject version guarantor | doc. Mgr. Eva Pertile, Ph.D. |
| Study level | undergraduate or graduate | | |
| | Study language | Czech |
| Year of introduction | 2007/2008 | Year of cancellation | 2014/2015 |
| Intended for the faculties | HGF | Intended for study types | Bachelor |
Subject aims expressed by acquired skills and competences
The main objective of lectures is to create a compact picture about modern analytical chemistry. Lectures are conceived so that students get an overview of general parts of analytical chemistry, which serves as a necessity to understand the laws of chemistry and reaction analysis and then is followed with an overview of various groups of practical activities, leading to the determination of the reference analyte.
Teaching methods
Lectures
Experimental work in labs
Other activities
Summary
The aim of the course is to broaden and deepen knowledge of chemical laboratory techniques including gaining practical skills. The aim of the course is to deepen students' readiness to solve analytic problems by selected instrumental methods. Provide an overview of selected analytical methods and procedures used to analyze real environmental samples and chemometric evaluation of analytical results.
Compulsory literature:
Recommended literature:
Additional study materials
Way of continuous check of knowledge in the course of semester
Knowledge achieved during the semester is revised in each practical training (laboratory exeercise). At the beginning of each laboratory exercise the student has to pass an entrance test, which contains brief questions on a laboratory task that he/she is supposed to perform that day. The test also contains questions related to labour safety and laboratory practice.
E-learning
Other requirements
Active participation in lectures and seminars.
Prerequisities
Subject has no prerequisities.
Co-requisities
Subject has no co-requisities.
Subject syllabus:
1. Defining the problem. Basic concepts. Processes of obtaining information. The basic division of analytical methods.
2. Basic units and concentration calculations. Types of interactions. Chemical equilibrium in analytical chemistry. Appraisal reactions.
3. The process of analysis - the general outline: the role of formulation and solution design, sampling, sample treatment before analysis, separation and concentration steps, their measurement, evaluation of measurement of results of analysis, design work resulting from the data obtained.
4. Sampling, sampling guidelines. Standard operating procedures for sampling, system QA / QC.
5. Basic parameters of sampling abiotic and biotic matrices. Sampling of air, including passive sampling.
6. Sampling of drinking and surface water, sediment sampling, groundwater and waste watersampling, sampling of sewage water, sampling of pedosphere.
7. Sampling of biotic matrices. Waste sampling.
8. Sample treatment prior to analysis. Non-destructive analytical methods. Destructive analytical methods: dissolution of samples and sample analysis, agents of mineralization.
9. Separation and concentration steps: elimination of interferents with chemical methods (masking) and physical separations. Separation methods: extraction, ion-exchange. The effectiveness of a separation process.
10. Evaluation of analytical results and procedures and expressing analytical results. Proposal for actions arising from the data obtained. Principles of good laboratory practice. Introduction to chemometrics.
11. Basics of qualitative analysis: classical system of separation of cations - hydrogen sulphide system, analytical classes of anions. Other criteria for determining anions. Evidence of hetero-organic substances.
12. Overview of basic analytical methods and their general characteristics: methods based on weighing, measuring volume. Gravimetric analysis: the principle of gravimetry, analytical use. Basic steps of analysis: sample preparation, precipitation, aging of the precipitate, washing and filtration, drying and annealing, weighing
clots calculating the concentration.
13. Quantitative chemical analysis. Volumetric analysis (volumetry): general procedure for determining, detecting the end point of the titration, titration curves, the calculation of the analyte from the titration data. Types of volumetric determinations and their applications: acid-base titration, precipitation titration, complexometric titration, redox titration. The principle analytical use.
14. Trends in analytical chemistry - mobile analytics.
Exercises syllabus:
Laboratory exercises supplies lectures and accentuates the acquisition of basic
operations of chemical analysis. All laboratory work must be carried out
safely, that is why students will get familiar with the labour safety in the laboratory at the beginning of the course. The objective of exercises is to acquaint students with classical methods of quantitative analysis.
Conditions for subject completion
Conditions for completion are defined only for particular subject version and form of study
Occurrence in study plans
Occurrence in special blocks
Assessment of instruction