460-6023/01 – Central Dogma of Molecular Biology (CDMB)

Gurantor departmentDepartment of Computer ScienceCredits10
Subject guarantordoc. Dr. Ing. Eva KriegováSubject version guarantordoc. Dr. Ing. Eva Kriegová
Study levelpostgraduateRequirementChoice-compulsory type B
YearSemesterwinter + summer
Study languageCzech
Year of introduction2019/2020Year of cancellation
Intended for the facultiesFEIIntended for study typesDoctoral
Instruction secured by
LoginNameTuitorTeacher giving lectures
DYS0006 Mgr. Tereza Dýšková, Ph.D.
FIL0152 Ing. Regina Fillerová, Ph.D.
FUR0029 Mgr. Jana Fürstová
KRI0203 doc. Dr. Ing. Eva Kriegová
MIK0424 Mgr. Zuzana Mikulková, Ph.D.
SCH0341 Mgr. Petra Schneiderová
Extent of instruction for forms of study
Form of studyWay of compl.Extent
Full-time Examination 28+0
Part-time Examination 28+0

Subject aims expressed by acquired skills and competences

The aim of the course is to deepen students' knowledge in the field of molecular biology, especially in understanding the of terminology and biological processes. In addition, this knowledge and skills will be further enhanced in a direction.

Teaching methods

Seminars
Individual consultations
Project work
Other activities

Summary

The subject is focused on the central dogma of molecular biology explaining that DNA molecule codes for RNA, which codes for proteins. Students will learn about the biological macromolecules responsible for the precise duplication/transcription/translation of the genetic information when the cell grows, divides, die. The subject covers also the most important experimental molecular techniques, bases of clinical genetics and application of genetic engineering in medicine and biology.

Compulsory literature:

• Bruce A. et al. Molecular Biology of the Cell, 6th Edition (2014), Garland Science, New York and Abingdon, UK • Watson J. D. et al. Molecular Biology of the Gene, 7th Edition (2014), Cold Spring Harbor Laboratory Press, New York

Recommended literature:

• Lodish H. et al. Molecular Cell Biology, 8th Edition (2016), W. H. Freeman and Co., New York

Way of continuous check of knowledge in the course of semester

Continuous monitoring of study activities and assigned tasks during regular consultations. If some publication activity will be a part of the student's tasks, the relevant article would be presented in the course.

E-learning

Other requirements

The student prepares and presents the work on a given topic.

Prerequisities

Subject has no prerequisities.

Co-requisities

Subject has no co-requisities.

Subject syllabus:

• Model organisms used in molecular biology, definition of concepts - inheritance, genome, gene, non-genic DNA, primary and secondary structure of DNA, palindromes, DNA denaturation and renaturation • Prokaryotic and eukaryotic genome - characteristics and structure • Replication of DNA in prokaryotes and eukaryotes - single phases, overview of replication apparatus enzymes • Cell cycle - molecular mechanisms of regulation, tumor transformation, cell death • Mutation - gene, chromosome and genome; spontaneous and induced • Repairs - repartition of reparation mechanisms; general (homologous) recombination • Characteristics of RNA, transcription and post-transcriptional modifications of RNA in prokaryotes and eukaryotes • Translations and post-translational modifications of proteins - proteosynthetic apparatus, individual phases, types of protein modifications • Regulation of gene expression in prokaryotes and eukaryotes • Methods of molecular biology • Enzymes experimentally used in molecular biology - polymerases, exonucleases, endonucleases, enzyme-modifying, ligase • Polymerase chain reaction (PCR) - principle, individual phases, PCR modifications, nucleic acid quantification by PCR • Sequencing of DNA; hybridization techniques - principles of methods • Cloning - principle, preparation of recombinant DNA molecule, recombinant molecule transfer methods, selection of recombinant DNA clones • Complex molecular biology in the post-genomic period - genome, transcript, proteome, metabolite; DNA chips; basics of proteomics (2D electrophoresis, mass spectrometry) • Genetically Related Diseases - Types of Inheritance, Overview of Significant Monogenic Inherited Diseases • Indirect and direct molecular genetic diagnostics - principles, methods overview • Clinical genetics - congenital developmental defects, genetic counseling • Gene therapy - definition and classification, gene therapy of cancer, gene therapy mediated oligonucleotides, gene immunization

Conditions for subject completion

Full-time form (validity from: 2019/2020 Winter semester)
Task nameType of taskMax. number of points
(act. for subtasks)
Min. number of points
Examination Examination  
Mandatory attendence parzicipation:

Show history

Occurrence in study plans

Academic yearProgrammeField of studySpec.ZaměřeníFormStudy language Tut. centreYearWSType of duty
2021/2022 (P0588D140003) Bioinformatics and Computational Biology K Czech Ostrava Choice-compulsory type B study plan
2021/2022 (P0588D140003) Bioinformatics and Computational Biology P Czech Ostrava Choice-compulsory type B study plan
2020/2021 (P0588D140003) Bioinformatics and Computational Biology P Czech Ostrava Choice-compulsory type B study plan
2020/2021 (P0588D140003) Bioinformatics and Computational Biology K Czech Ostrava Choice-compulsory type B study plan

Occurrence in special blocks

Block nameAcademic yearForm of studyStudy language YearWSType of blockBlock owner