460-6023/02 – Central Dogma of Molecular Biology (CDMB)
| Gurantor department | Department of Computer Science | Credits | 10 |
| Subject guarantor | doc. Dr. Ing. Eva Kriegová | Subject version guarantor | doc. Dr. Ing. Eva Kriegová |
| Study level | postgraduate | Requirement | Choice-compulsory type B |
| Year | | Semester | winter + summer |
| | Study language | English |
| Year of introduction | 2019/2020 | Year of cancellation | |
| Intended for the faculties | FEI | Intended for study types | Doctoral |
Subject aims expressed by acquired skills and competences
The aim of the course is to deepen students' knowledge in the field of molecular biology, especially in understanding the of terminology and biological processes. In addition, this knowledge and skills will be further enhanced in a direction.
Teaching methods
Seminars
Individual consultations
Project work
Other activities
Summary
The subject is focused on the central dogma of molecular biology explaining that DNA molecule codes for RNA, which codes for proteins. Students will learn about the biological macromolecules responsible for the precise duplication/transcription/translation of the genetic information when the cell grows, divides, die. The subject covers also the most important experimental molecular techniques, bases of clinical genetics and application of genetic engineering in medicine and biology.
Compulsory literature:
• Bruce A. et al. Molecular Biology of the Cell, 6th Edition (2014), Garland Science, New York and Abingdon, UK
• Watson J. D. et al. Molecular Biology of the Gene, 7th Edition (2014), Cold Spring Harbor Laboratory Press, New York
Recommended literature:
• Lodish H. et al. Molecular Cell Biology, 8th Edition (2016), W. H. Freeman and Co., New York
Additional study materials
Way of continuous check of knowledge in the course of semester
Continuous monitoring of study activities and assigned tasks during regular consultations. If some publication activity will be a part of the student's tasks, the relevant article would be presented in the course.
Oral exam.
E-learning
Other requirements
The student prepares and presents the work on a given topic.
Prerequisities
Subject has no prerequisities.
Co-requisities
Subject has no co-requisities.
Subject syllabus:
• Model organisms used in molecular biology, definition of concepts - inheritance, genome, gene, non-genic DNA, primary and secondary structure of DNA, palindromes, DNA denaturation and renaturation
• Prokaryotic and eukaryotic genome - characteristics and structure
• Replication of DNA in prokaryotes and eukaryotes - single phases, overview of replication apparatus enzymes
• Cell cycle - molecular mechanisms of regulation, tumor transformation, cell death
• Mutation - gene, chromosome and genome; spontaneous and induced
• Repairs - repartition of reparation mechanisms; general (homologous) recombination
• Characteristics of RNA, transcription and post-transcriptional modifications of RNA in prokaryotes and eukaryotes
• Translations and post-translational modifications of proteins - proteosynthetic apparatus, individual phases, types of protein modifications
• Regulation of gene expression in prokaryotes and eukaryotes
• Methods of molecular biology
• Enzymes experimentally used in molecular biology - polymerases, exonucleases, endonucleases, enzyme-modifying, ligase
• Polymerase chain reaction (PCR) - principle, individual phases, PCR modifications, nucleic acid quantification by PCR
• Sequencing of DNA; hybridization techniques - principles of methods
• Cloning - principle, preparation of recombinant DNA molecule, recombinant molecule transfer methods, selection of recombinant DNA clones
• Complex molecular biology in the post-genomic period - genome, transcript, proteome, metabolite; DNA chips; basics of proteomics (2D electrophoresis, mass spectrometry)
• Genetically Related Diseases - Types of Inheritance, Overview of Significant Monogenic Inherited Diseases
• Indirect and direct molecular genetic diagnostics - principles, methods overview
• Clinical genetics - congenital developmental defects, genetic counseling
• Gene therapy - definition and classification, gene therapy of cancer, gene therapy mediated oligonucleotides, gene immunization
Conditions for subject completion
Occurrence in study plans
Occurrence in special blocks
Assessment of instruction
Předmět neobsahuje žádné hodnocení.