546-0116/01 – Microbiology Practicum (MBP)

Gurantor departmentDepartment of Environmental EngineeringCredits4
Subject guarantordoc. Mgr. Hana Vojtková, Ph.D.Subject version guarantordoc. Mgr. Hana Vojtková, Ph.D.
Study levelundergraduate or graduateRequirementCompulsory
Year1Semesterwinter
Study languageCzech
Year of introduction2019/2020Year of cancellation
Intended for the facultiesHGFIntended for study typesFollow-up Master
Instruction secured by
LoginNameTuitorTeacher giving lectures
VOJ195 doc. Mgr. Hana Vojtková, Ph.D.
Extent of instruction for forms of study
Form of studyWay of compl.Extent
Full-time Credit 0+4
Part-time Credit 0+16

Subject aims expressed by acquired skills and competences

Target competences lie in gaining new knowledge and its application in observation, experiments and communication: - to characterize the morphological characteristics of microorganisms, to understand phylogenetic relations of the different groups, to learn about and to term characteristic organisms of the individual taxons and to understand the significance of pathogenic as well as non-pathogenic species - to apply the gained knowledge in dealing with practical tasks in seminars, to consider the consequences of a phenomenon from the environmental, sanitary as well as economic points of view; to build a positive attitude to nature - to be able to work independently on the set tasks, to observe and objectively record the experiment results, to adhere to safe (aseptic) work principles when working with biological material and technical equipment - to be able to work with expert/academic texts, to be familiar with the expert terminology, to be able to synthetize the obtained experimental data and to justify own approach in dealing with a biological problem

Teaching methods

Experimental work in labs
Other activities

Summary

The subject is focused on consolidation and broadening of microbiological laboratory skills enabling isolation, cultivation and identification of microorganisms from waste and other environmental samples. Learning outcomes of the subject are to deepen the knowledge and skills of students in working with microorganisms including the application of basic laboratory procedures, methods and techniques used in microbiological analyses of waste samples, including the identification of significant indicator groups of microorganisms and verification of the presence of significant pathogens.

Compulsory literature:

MORELLO, Josephine A., Paul A. GRANATO a Helen ECKEL MIZER. Laboratory Manual and Workbook in Microbiology. McGraw-Hill Science, 2003. ISBN 972-0-0724-6354-6 BURDASS, Dariel, John GRAINGER a Janet HURST. Basic Practical Microbiology: A Manual. Society for General Microbiology, 2006. ISBN 0-95368-383-4 GOLDMAN, Emanuel a Lorrence H. GREEN. Practical Handbook of Microbiology. CRC Press, Taylor & Francis Group, 2015. ISBN 978-1-4665-8739-7 KUMAR, Surinder. Textbook of Microbiology. Jaypee Brothers Medical Publishers Ltd., 2012. ISBN 978-93-5025-510-0

Recommended literature:

BITTON, Gabriel. Wastewater Microbiology. John Wiley & Sons, 2011. ISBN 978-0-470-63033-4 PEPPER,Ian, Charles GERBA a Terry GENTRY. Environmental Microbiology. Academic Press, 2015. ISBN 978-0-123-94626-3 BIOLOG - Microbial Identification & Characterization (www.biolog.com) List of Prokaryotic Names with Standing in Nomenclature (http://www.bacterio.net) Species lists in the data base «Species fungorum» (http://www.speciesfungorum.org)

Way of continuous check of knowledge in the course of semester

Practical skills are continuously checked when students work at tutorials. The subject is concluded with a credit, students will submit reports from tutorials with elaborated tasks. The credit is awarded on condition of 100% attendance at tutorials, elaborating and submitting reports, including completed assignments, and passing the credit test at 70 % at a minimum.

E-learning

Other requirements

- regular participation at tutorials (attendance is compulsory and is checked by the teacher) - elaboration of reports and assigned tasks - passing the credit test at 70 % at a minimum

Prerequisities

Subject has no prerequisities.

Co-requisities

Subject has no co-requisities.

Subject syllabus:

1. Principles of safe work in a microbiological laboratory 2. Equipment of a microbiological laboratory, basics of aseptic work 3. Preparation of nutrient media and their sterilization, conditions of cultivation of microorganisms 4. Bacteria and their morphological features on nutrient media 5. Microscopic filamentous fungi and their morphology on nutrient media 6. Yeast and yeast microorganisms, their morphological features 7. Cyanobacteria, microscopic algae and their morphology 8. Sampling and preparation of samples for microbiological analysis 9. Cultivation methods for determining the number of microorganisms in a sample 10. Microscopic methods for determining the number of microorganisms in a sample 11. Indirect methods for determining the number of microorganisms in a sample 12. Indicator microorganisms and identification of basic pathogens 13. Other methods of microbiological analysis of waste samples 14. Protocol checking, credit test

Conditions for subject completion

Part-time form (validity from: 2020/2021 Winter semester)
Task nameType of taskMax. number of points
(act. for subtasks)
Min. number of points
Credit Credit 100 (100) 51
        Závěrečný test Written test 100  51
Mandatory attendence parzicipation: - regular participation at tutorials (attendance is compulsory and is checked by the teacher) - elaboration of reports and assigned tasks - passing the credit test

Show history

Occurrence in study plans

Academic yearProgrammeField of studySpec.ZaměřeníFormStudy language Tut. centreYearWSType of duty
2021/2022 (N0788A290001) Waste Management and Mineral Processing P Czech Ostrava 1 Compulsory study plan
2021/2022 (N0788A290001) Waste Management and Mineral Processing K Czech Ostrava 1 Compulsory study plan
2021/2022 (N0788A290001) Waste Management and Mineral Processing K Czech Most 1 Compulsory study plan
2020/2021 (N0788A290001) Waste Management and Mineral Processing K Czech Most 1 Compulsory study plan
2020/2021 (N0788A290001) Waste Management and Mineral Processing K Czech Ostrava 1 Compulsory study plan
2020/2021 (N0788A290001) Waste Management and Mineral Processing P Czech Ostrava 1 Compulsory study plan

Occurrence in special blocks

Block nameAcademic yearForm of studyStudy language YearWSType of blockBlock owner