546-0116/01 – Microbiology Practicum (MBP)
| Gurantor department | Department of Environmental Engineering | Credits | 4 |
| Subject guarantor | doc. Mgr. Hana Vojtková, Ph.D. | Subject version guarantor | doc. Mgr. Hana Vojtková, Ph.D. |
| Study level | undergraduate or graduate | Requirement | Compulsory |
| Year | 1 | Semester | winter |
| | Study language | Czech |
| Year of introduction | 2019/2020 | Year of cancellation | |
| Intended for the faculties | HGF | Intended for study types | Follow-up Master |
Subject aims expressed by acquired skills and competences
Target competences lie in gaining new knowledge and its application in observation, experiments and communication:
- to characterize the morphological characteristics of microorganisms, to understand phylogenetic relations of the different groups, to learn about and to term characteristic organisms of the individual taxons and to understand the significance of pathogenic as well as non-pathogenic species
- to apply the gained knowledge in dealing with practical tasks in seminars, to consider the consequences of a phenomenon from the environmental, sanitary as well as economic points of view; to build a positive attitude to nature
- to be able to work independently on the set tasks, to observe and objectively record the experiment results, to adhere to safe (aseptic) work principles when working with biological material and technical equipment
- to be able to work with expert/academic texts, to be familiar with the expert terminology, to be able to synthetize the obtained experimental data and to justify own approach in dealing with a biological problem
Teaching methods
Tutorials
Experimental work in labs
Other activities
Field data collection
Summary
The subject is focused on consolidation and broadening of microbiological laboratory skills enabling isolation, cultivation and identification of microorganisms from waste and other environmental samples.
Learning outcomes of the subject are to deepen the knowledge and skills of students in working with microorganisms including the application of basic laboratory procedures, methods and techniques used in microbiological analyses of waste samples, including the identification of significant indicator groups of microorganisms and verification of the presence of significant pathogens.
Compulsory literature:
Recommended literature:
Additional study materials
Way of continuous check of knowledge in the course of semester
Practical skills are continuously checked when students work at tutorials.
The subject is concluded with a credit, students will submit reports from tutorials with elaborated tasks.
The credit is awarded on condition of 100% attendance at tutorials, elaborating and submitting reports, including completed assignments, and passing the credit test at 51 % at a minimum.
E-learning
Other requirements
- regular participation at tutorials (attendance is compulsory and is checked by the teacher)
- elaboration of reports and assigned tasks
- passing the credit test at 70 % at a minimum
Prerequisities
Subject has no prerequisities.
Co-requisities
Subject has no co-requisities.
Subject syllabus:
1. Principles of safe work in a microbiological laboratory
2. Equipment of a microbiological laboratory, basics of aseptic work
3. Preparation of nutrient media and their sterilization, conditions of cultivation of microorganisms
4. Bacteria and their morphological features on nutrient media
5. Microscopic filamentous fungi and their morphology on nutrient media
6. Yeast and yeast microorganisms, their morphological features
7. Cyanobacteria, microscopic algae and their morphology
8. Sampling and preparation of samples for microbiological analysis
9. Cultivation methods for determining the number of microorganisms in a sample
10. Microscopic methods for determining the number of microorganisms in a sample
11. Indirect methods for determining the number of microorganisms in a sample
12. Indicator microorganisms and identification of basic pathogens
13. Other methods of microbiological analysis of waste samples
Conditions for subject completion
Occurrence in study plans
Occurrence in special blocks
Assessment of instruction