546-0354/02 – Instrumental Methods of Analysis (IMA)
Gurantor department | Department of Environmental Engineering | Credits | 6 |
Subject guarantor | doc. Mgr. Eva Pertile, Ph.D. | Subject version guarantor | doc. Mgr. Eva Pertile, Ph.D. |
Study level | undergraduate or graduate | Requirement | Compulsory |
Year | 2 | Semester | summer |
| | Study language | Czech |
Year of introduction | 2006/2007 | Year of cancellation | 2008/2009 |
Intended for the faculties | HGF | Intended for study types | Bachelor |
Subject aims expressed by acquired skills and competences
Obtaining basic knowledge not only about environmental sampling in relation to its share in the overall uncertainty of environmental analyzes but also in instrumental analytical methods. This will increase student readiness to solve analytic problems by selected instrumental methods.
Obtaining the competence to work in an analytical laboratory with basic instrumental equipment, the ability to present and evaluate the results, to learn the correct principles of working with the sample.
Teaching methods
Lectures
Experimental work in labs
Terrain work
Other activities
Summary
The lecture provides information about selected modern instrumental methods of research and monitoring of the environment, its use and application. During the lectures, students will get acquainted with the principles of analytical methods that are commonly used in control and development analytical laboratories.
Compulsory literature:
Recommended literature:
Way of continuous check of knowledge in the course of semester
The every laboratory starts with 5-minute 6-question test, to prove that student read the manual and is able to work safely and purposefully. The 3 questions need to be answered correctly to be allowed to work. If not the test is classified “0b”. Every work is classified after delivering of report or the not classified if report is not delivered or delivered late.
E-learning
Other requirements
Prerequisities
Subject has no prerequisities.
Co-requisities
Subject has no co-requisities.
Subject syllabus:
1. Problems delimitation. Basic concepts. Processes obtaining information. Analytical methods absolute and comparative. Calibration methods. Developments in instrumentation methods of analyses.
2. - 3. Results classification of analyses and way their explication. Statistical interpretation of analytical results. Referential material, circular test. Principles of chemometric accesses and methods. Testing of metrological results properties.
4. Fundamental principles of electroanalytical methods and their classification. Theoretic principles.
5. Electroanalytical methods: methods based on measuring of potential (equilibrium potentiometry, potentiometric titrations). Axioms, instrumentation, utilization, advantages and disavantages.
6. Electroanalytical methods: methods based on current (voltammetry and polarography, coulometry, electrogravimetry) and conductivity (conductometry). Axioms, instrumentation, utilization, advantages and disavantages.
7. Optical analytical methods: theoretic principles. Optical spectral apparatus. Methods without exchange of energy (refractometry, polarimetry, nephelometry). Axioms, instrumentation, utilization, advantages and disavantages.
8. - 9. Emissive atomic spectral frequency bands. Flame photometer photometry. Atomic absorptive spectrometric: UV/Vis spectrometric, luminescent analysis. Principles theory, instrumentation, qualitative and quantitative aspects, application, analytical utilization. Automatic analyzers with optical detection: FIA, CFA.
10. Infra - red spectrometric - method axiom, instrumentation, analytical utilization. Raman spectra - method axiom , analytical utilization.
11. Separative and concentration steps: theory. Separative methods:classification according to axiom and according to experimental ordering, operation separative of the process. Extraction.
12. Diaphragm separation. Mass spectrometry. Bases theory, instrumentation, qualitative and quantitative aspects, application.
13. Chromatographic methods. Planar chromatography: Chromatography on thin layer, bases theory, instrumentation, qualitative and quantitative aspects, application.
11. Column chromatography: Liquid chromatography. Gas chromatography. Theory, bases instrumentation, qualitative and quantitative characteristics, analytical utilization.
14. Electromigration methods: theory, zonal and capillary electrophoresis, isotachophoresis. Bases instrumentation, qualitative and quantitative characteristics, analytical utilization.
Conditions for subject completion
Occurrence in study plans
Occurrence in special blocks
Assessment of instruction
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