546-0354/03 – Instrumental Methods of Analysis (IMA)

Gurantor departmentDepartment of Environmental EngineeringCredits8
Subject guarantordoc. Mgr. Eva Pertile, Ph.D.Subject version guarantordoc. Mgr. Eva Pertile, Ph.D.
Study levelundergraduate or graduate
Study languageCzech
Year of introduction2006/2007Year of cancellation
Intended for the facultiesHGFIntended for study typesBachelor
Instruction secured by
LoginNameTuitorTeacher giving lectures
PER34 doc. Mgr. Eva Pertile, Ph.D.
Extent of instruction for forms of study
Form of studyWay of compl.Extent
Full-time Credit and Examination 2+4

Subject aims expressed by acquired skills and competences

KNOWLEDGE KNOWLEDGE OF THE SUBJECT: Obtaining basic knowledge not only about environmental sampling in relation to its share in the overall uncertainty of environmental analyzes but also in instrumental analytical methods. This will increase student readiness to solve analytic problems by selected instrumental methods. COMPETENCES PROCESSED AFTER THE END OF THE SUBJECT: Obtaining the competence to work in an analytical laboratory with basic instrumental equipment, the ability to present and evaluate the results, to learn the correct principles of working with the sample.

Teaching methods

Lectures
Experimental work in labs
Terrain work
Other activities

Summary

The lecture provides clear information on selected advanced instrumental methods of environmental research and monitoring, theoretical basics, instrumental technique, its use and application. Emphasis is placed on explanation of principles, technical equipment and utilization of electrochemical, separation and optical methods. In the course of the lectures the students will get acquainted with the principles of analytical methods, which are commonly used in control and development analytical laboratories. The range of methods discussed includes both classical analysis methods and instrumental methods that make up most of the subject's content.

Compulsory literature:

PERTILE, E. Instrumental Methods of Analysis. Ostrava: Vysoká škola báňská - Technická univerzita Ostrava, 2017. ISBN 978-80-248-4124-3.

Recommended literature:

ROUESSAC Francis and ROUESSAC, Annick: Chemical Analysis Modern Instrumentation Methods and Techniques. March 2007. 600 pp. ISBN: 978-0-470-85903-2. NOLLET M., L., Chromatographic Analysis of the Environment, Chromatographic Science Series, Volume 93, 1297 s. 2006, ISBN 0-8247-2629-4.

Way of continuous check of knowledge in the course of semester

Vědomosti studenta jsou ověřovány písemnou formou, přičemž je kladen důraz na porozumění danému tématu a aplikaci teoretických poznatků při řešení praktických úloh. Hodnocení zahrnuje i kolektivní hodnocení a sebehodnocení. Do celkového hodnocení je zahrnuta i aktivní práce studentů v hodině. Každá laboratoř začíná 5ti minutovým 6ti otázkovým testem, kterým student prokazuje, že četl návody a je schopen pracovat bezpečně a účelně.

E-learning

Další požadavky na studenta

Active participation in the lectures and the seminars, demonstration of successful processing subtasks, entered continuously in the semester.

Prerequisities

Subject has no prerequisities.

Co-requisities

Subject has no co-requisities.

Subject syllabus:

1. Definition of the issue. Information-gathering process. The analysis process. Evaluation of analytical results and practices, expression analysis results. Statistical results. Draft action arising from the data obtained. Principles of Good Laboratory Practice. Basics of chemometrics. 2. Sampling, the general principle and sources of the errors. Standard operating procedures for sampling; QA / QC system. Basic parameters sampling of abiotic and biotic matrices. 3. Treatment of the sample before analysis. Nondestructive methods. Destructive methods. Separation and concentration steps. 4. Qualitative analysis: classical system of dividing cations. Evidence heteroatoms in organic compounds. 5. Overview of the basic analytical methods and their general properties: methods based on weighing, measuring volume. The principles of individual methods; instrumentation; application in the analysis of environmental components; advantages/disadvantages. 6. An introduction to the instrumental methods. Use of both qualitative and quantitative. Definition of the issue. The analytical methods absolute and comparative. Rapid analytical methods - screening. Intended use. Advantages, disadvantages. Calibration methods. Trends in the instrumental methods of analysis. 7. Electrochemical methods based on the voltage measurement. Electrochemical methods based on the current measurement. The principles of individual methods; instrumentation; application in the analysis of environmental components; advantages/disadvantages. 8. Summary separation methods: Planar chromatography: TLC + HPTLC, PC, advantages/disadvantages. 9. Column chromatography: GC + HPLC. New trends: UHPLC, GC / MS, HPLC / MS. The principles of individual methods; instrumentation; application in the analysis of environmental components; advantages/disadvantages. 10. Electrophoresis. Mass spectrometry. The principles of individual methods; instrumentation; application in the analysis of environmental components; advantages/disadvantages. 11. Overview of the optical methods. Optical spectral device. Refractometry, interferometry, polarimetry and turbidimetry: Principles of the individual methods; instrumentation; application in the analysis of the environmental components; advantages/disadvantages. 12. Atomic emission spectral analysis. Flame photometry. Atomic absorption spectrometry. UV / Vis spectrometry. The principles of individual methods; instrumentation; application in the analysis of environmental components; advantages/disadvantages. 13. Luminescence analysis. Infrared spectrometry. Raman spectra; X-ray fluorescence spectrometry. Radiochemical methods. Understanding the various methods, instrumentation, application in the analysis of environmental components; advantages/disadvantages. 14. New trends in instrumental methods with applications in biotechnology.

Conditions for subject completion

Full-time form (validity from: 2016/2017 Winter semester)
Task nameType of taskMax. number of points
(act. for subtasks)
Min. number of points
Credit and Examination Credit and Examination 100 (100) 51
        Credit Credit 33 (33) 20
                Vstupní test Written test 4  2
                Protokoly Laboratory work 8  5
                Písemka Written test 5  3
                Zápočtový test Written test 13  8
                Aktivita Laboratory work 3  2
        Examination Examination 67  31
Mandatory attendence parzicipation:

Show history

Occurrence in study plans

Academic yearProgrammeField of studySpec.FormStudy language Tut. centreYearWSType of duty
2019/2020 (B2102) Mineral Raw Materials (3904R028) Environmental Biotechnology P Czech Ostrava 2 Compulsory study plan
2018/2019 (B2102) Mineral Raw Materials (3904R028) Environmental Biotechnology P Czech Ostrava 2 Compulsory study plan
2017/2018 (B2102) Mineral Raw Materials (3904R028) Environmental Biotechnology P Czech Ostrava 2 Compulsory study plan
2016/2017 (B2102) Mineral Raw Materials (3904R028) Environmental Biotechnology P Czech Ostrava 2 Compulsory study plan
2015/2016 (B2102) Mineral Raw Materials (3904R028) Environmental Biotechnology P Czech Ostrava 2 Compulsory study plan
2014/2015 (B2102) Mineral Raw Materials (3904R028) Environmental Biotechnology P Czech Ostrava 2 Compulsory study plan
2013/2014 (B2102) Mineral Raw Materials (3904R028) Environmental Biotechnology P Czech Ostrava 2 Compulsory study plan
2012/2013 (B2102) Mineral Raw Materials (3904R028) Environmental Biotechnology P Czech Ostrava 2 Compulsory study plan
2011/2012 (B2102) Mineral Raw Materials (3904R028) Environmental Biotechnology P Czech Ostrava 2 Compulsory study plan
2010/2011 (B2102) Mineral Raw Materials (3904R028) Environmental Biotechnology P Czech Ostrava 2 Compulsory study plan
2009/2010 (B2102) Mineral Raw Materials (3904R028) Environmental Biotechnology P Czech Ostrava 2 Compulsory study plan
2008/2009 (B2102) Mineral Raw Materials (3904R028) Environmental Biotechnology P Czech Ostrava 2 Compulsory study plan
2007/2008 (B2102) Mineral Raw Materials (3904R028) Environmental Biotechnology P Czech Ostrava 2 Compulsory study plan

Occurrence in special blocks

Block nameAcademic yearForm of studyStudy language YearWSType of blockBlock owner
ECTS - FMG 2017/2018 Full-time English Optional 501 - Study Office stu. block
ECTS - FMG 2016/2017 Full-time English Optional 501 - Study Office stu. block
O - ECTS FMG 2015/2016 Full-time Czech Optional 501 - Study Office stu. block
O - ECTS FMG - Bc. 2014/2015 Full-time Czech Optional 501 - Study Office stu. block
O - ECTS FMG - Bc. 2013/2014 Full-time Czech Optional 501 - Study Office stu. block
O - ECTS FMG - Bc. 2012/2013 Full-time Czech Optional 501 - Study Office stu. block