546-0354/04 – Instrumental Methods of Analysis (IMA)
| Gurantor department | Department of Environmental Engineering | Credits | 5 |
| Subject guarantor | doc. Mgr. Eva Pertile, Ph.D. | Subject version guarantor | doc. Mgr. Eva Pertile, Ph.D. |
| Study level | undergraduate or graduate | Requirement | Compulsory |
| Year | 1 | Semester | summer |
| | Study language | Czech |
| Year of introduction | 1999/2000 | Year of cancellation | 2008/2009 |
| Intended for the faculties | HGF | Intended for study types | Bachelor |
Subject aims expressed by acquired skills and competences
Obtaining basic knowledge not only about environmental sampling in relation to its share in the overall uncertainty of environmental analyzes but also in instrumental analytical methods. This will increase student readiness to solve analytic problems by selected instrumental methods.
Obtaining the competence to work in an analytical laboratory with basic instrumental equipment, the ability to present and evaluate the results, to learn the correct principles of working with the sample.
Teaching methods
Lectures
Experimental work in labs
Terrain work
Other activities
Summary
The lecture provides information about selected modern instrumental methods of research and monitoring of the environment, its use and application. During the lectures, students will get acquainted with the principles of analytical methods that are commonly used in control and development analytical laboratories.
Compulsory literature:
Recommended literature:
Way of continuous check of knowledge in the course of semester
Knowledge acquired in first block of practice will be tested by control test. Departure from second block will be elaborate protocol.
E-learning
Other requirements
Prerequisities
Subject has no prerequisities.
Co-requisities
Subject has no co-requisities.
Subject syllabus:
1. Problems specification. Basic concept. Interaktion types. Chemical equiponderances in analytical chemistry. Classification of analytical reaction.
2. Process of analyse. Sampling and sample preparation, separation processes. search result evaluation. GLP.
3. Developments in analytical chemistry - new instrumental methodology, mobile analytical chemistry.
4. Quantitative analysis. Principle of gravity surveying, analytical development. Volumetric analysis.
5. Electroanalytical methods analysis: Potentiometry.
6. Electroanalytical methods analysis: Voltametry and polarography. conductometry.
7. View of separative methods, axioms and their analytical usage. Planar chromatograpghy /TLC, HPTLC, PC)
8. Column chromatography, axioms and classification, instrumentation, kvalitation and kvantitation analysis, chromatogram, analytical usage.
9. Extraction: LLE, SFE, SPE, SPME
10. Electrophoresis: axioms, instumentation, analytical usage. IEF, CE. Membrane separation: ultrafiltration, dialysis, reverse osmose, electrodialysis. Mass spectrometry.
11. Optical methods. Optical spectral instruments. Refractometry, interferometry, polarimetry, turbidimetry, axioms, instumentation, analytical usage.
12. Emission atomic spectral analysis. flame photometry. Atomic absorption spectrometry. UV/Vis spectrometry. Axioms, instumentation, analytical usage.
13. Infrared spectrometry. Raman spectra - axioms, instumentation, analytical usage.
14. RTG spectrometry - axioms, instumentation, analytical usage. Radiochemical methods.
Conditions for subject completion
Occurrence in study plans
Occurrence in special blocks
Assessment of instruction
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