546-0354/04 – Instrumental Methods of Analysis (IMA)

Gurantor departmentDepartment of Environmental EngineeringCredits5
Subject guarantordoc. Mgr. Eva Pertile, Ph.D.Subject version guarantordoc. Mgr. Eva Pertile, Ph.D.
Study levelundergraduate or graduateRequirementCompulsory
Study languageCzech
Year of introduction1999/2000Year of cancellation2008/2009
Intended for the facultiesHGFIntended for study typesBachelor
Instruction secured by
LoginNameTuitorTeacher giving lectures
NEZ022 Ing. Lucie Čablíková
PER34 doc. Mgr. Eva Pertile, Ph.D.
STE68 Ing. Radka Wolfová, Ph.D.
Extent of instruction for forms of study
Form of studyWay of compl.Extent
Full-time Credit and Examination 2+2
Combined Credit and Examination 12+0

Subject aims expressed by acquired skills and competences

KNOWLEDGE KNOWLEDGE OF THE SUBJECT: Obtaining basic knowledge not only about environmental sampling in relation to its share in the overall uncertainty of environmental analyzes but also in instrumental analytical methods. This will increase student readiness to solve analytic problems by selected instrumental methods. COMPETENCES PROCESSED AFTER THE END OF THE SUBJECT: Obtaining the competence to work in an analytical laboratory with basic instrumental equipment, the ability to present and evaluate the results, to learn the correct principles of working with the sample.

Teaching methods

Experimental work in labs
Terrain work
Other activities


The lecture provides clear information on selected advanced instrumental methods of environmental research and monitoring, theoretical basics, instrumental technique, its use and application. Emphasis is placed on explanation of principles, technical equipment and utilization of electrochemical, separation and optical methods. In the course of the lectures the students will get acquainted with the principles of analytical methods, which are commonly used in control and development analytical laboratories. The range of methods discussed includes both classical analysis methods and instrumental methods that make up most of the subject's content.

Compulsory literature:

PERTILE, E. Instrumental Methods of Analysis. Ostrava: Vysoká škola báňská - Technická univerzita Ostrava, 2017. ISBN 978-80-248-4124-3.

Recommended literature:

ROUESSAC Francis and ROUESSAC, Annick: Chemical Analysis Modern Instrumentation Methods and Techniques. March 2007. 600 pp. ISBN: 978-0-470-85903-2. NOLLET M., L., Chromatographic Analysis of the Environment, Chromatographic Science Series, Volume 93, 1297 s. 2006, ISBN 0-8247-2629-4.

Way of continuous check of knowledge in the course of semester

Knowledge acquired in first block of practice will be tested by control test. Departure from second block will be elaborate protocol.


Další požadavky na studenta


Subject has no prerequisities.


Subject has no co-requisities.

Subject syllabus:

1. Problems specification. Basic concept. Interaktion types. Chemical equiponderances in analytical chemistry. Classification of analytical reaction. 2. Process of analyse. Sampling and sample preparation, separation processes. search result evaluation. GLP. 3. Developments in analytical chemistry - new instrumental methodology, mobile analytical chemistry. 4. Quantitative analysis. Principle of gravity surveying, analytical development. Volumetric analysis. 5. Electroanalytical methods analysis: Potentiometry. 6. Electroanalytical methods analysis: Voltametry and polarography. conductometry. 7. View of separative methods, axioms and their analytical usage. Planar chromatograpghy /TLC, HPTLC, PC) 8. Column chromatography, axioms and classification, instrumentation, kvalitation and kvantitation analysis, chromatogram, analytical usage. 9. Extraction: LLE, SFE, SPE, SPME 10. Electrophoresis: axioms, instumentation, analytical usage. IEF, CE. Membrane separation: ultrafiltration, dialysis, reverse osmose, electrodialysis. Mass spectrometry. 11. Optical methods. Optical spectral instruments. Refractometry, interferometry, polarimetry, turbidimetry, axioms, instumentation, analytical usage. 12. Emission atomic spectral analysis. flame photometry. Atomic absorption spectrometry. UV/Vis spectrometry. Axioms, instumentation, analytical usage. 13. Infrared spectrometry. Raman spectra - axioms, instumentation, analytical usage. 14. RTG spectrometry - axioms, instumentation, analytical usage. Radiochemical methods.

Conditions for subject completion

Full-time form (validity from: 1960/1961 Summer semester)
Task nameType of taskMax. number of points
(act. for subtasks)
Min. number of points
Exercises evaluation and Examination Credit and Examination 100 (100) 51
        Exercises evaluation Credit 45 (45) 0
                Project Project 30  0
                Written exam Written test 15  0
        Examination Examination 55 (55) 0
                Written examination Written examination 30  0
                Oral Oral examination 25  0
Mandatory attendence parzicipation:

Show history

Occurrence in study plans

Academic yearProgrammeField of studySpec.FormStudy language Tut. centreYearWSType of duty
2007/2008 (B2102) Mineral Raw Materials (3904R005) Environmental Engineering P Czech Ostrava 1 Compulsory study plan
2006/2007 (B2102) Mineral Raw Materials (3904R005) Environmental Engineering P Czech Ostrava 1 Compulsory study plan
2006/2007 (B2102) Mineral Raw Materials (3904R005) Environmental Engineering K Czech Ostrava 1 Compulsory study plan

Occurrence in special blocks

Block nameAcademic yearForm of studyStudy language YearWSType of blockBlock owner