546-0354/05 – Instrumental Methods of Analysis (IMA)

Gurantor departmentDepartment of Environmental EngineeringCredits8
Subject guarantordoc. Mgr. Eva Pertile, Ph.D.Subject version guarantordoc. Mgr. Eva Pertile, Ph.D.
Study levelundergraduate or graduateRequirementCompulsory
Study languageCzech
Year of introduction2006/2007Year of cancellation2009/2010
Intended for the facultiesHGFIntended for study typesBachelor
Instruction secured by
LoginNameTuitorTeacher giving lectures
NEZ022 Ing. Lucie Čablíková
PER34 doc. Mgr. Eva Pertile, Ph.D.
HOL517 Ing. Barbora Poláková, Ph.D.
STE68 Ing. Radka Wolfová, Ph.D.
Extent of instruction for forms of study
Form of studyWay of compl.Extent
Full-time Credit and Examination 2+2
Part-time Credit and Examination 6+6

Subject aims expressed by acquired skills and competences

KNOWLEDGE KNOWLEDGE OF THE SUBJECT: Obtaining basic knowledge not only about environmental sampling in relation to its share in the overall uncertainty of environmental analyzes but also in instrumental analytical methods. This will increase student readiness to solve analytic problems by selected instrumental methods. COMPETENCES PROCESSED AFTER THE END OF THE SUBJECT: Obtaining the competence to work in an analytical laboratory with basic instrumental equipment, the ability to present and evaluate the results, to learn the correct principles of working with the sample.

Teaching methods

Experimental work in labs
Terrain work
Other activities


The lecture provides clear information on selected advanced instrumental methods of environmental research and monitoring, theoretical basics, instrumental technique, its use and application. Emphasis is placed on explanation of principles, technical equipment and utilization of electrochemical, separation and optical methods. In the course of the lectures the students will get acquainted with the principles of analytical methods, which are commonly used in control and development analytical laboratories. The range of methods discussed includes both classical analysis methods and instrumental methods that make up most of the subject's content.

Compulsory literature:

PERTILE, E. Instrumental Methods of Analysis. Ostrava: Vysoká škola báňská - Technická univerzita Ostrava, 2017. ISBN 978-80-248-4124-3.

Recommended literature:

ROUESSAC Francis and ROUESSAC, Annick: Chemical Analysis Modern Instrumentation Methods and Techniques. March 2007. 600 pp. ISBN: 978-0-470-85903-2. NOLLET M., L., Chromatographic Analysis of the Environment, Chromatographic Science Series, Volume 93, 1297 s. 2006, ISBN 0-8247-2629-4.

Way of continuous check of knowledge in the course of semester

Each laboratory exercise starts with a 5-minute 6-question test, to prove that the student read the manual (instructions to the laboratory exercise) and is able to work safely and purposefully. Three questions in the test need to be answered correctly to be allowed to work. If not the test is classified “0 points”. Each laboratory exercise is classified after delivering the final report or not classified if the report is not delivered or delivered late.


Other requirements


Subject has no prerequisities.


Subject has no co-requisities.

Subject syllabus:

1. Defining the problem. Basic concepts. Processes of obtaining information. Types of interactions. Chemical equilibrium in analytical chemistry. Appraisal reactions. 2. Process of analysis. Sampling and sample treatment before analysis, separation and concentration steps. Evaluation of analytical results and procedures and expression analysis results. Proposal for action arising from the data obtained. Principles of good laboratory practice. 3. Trends in analytical chemistry - new instrumental procedures, including mobile analytics. 4. Quantitative chemical analysis. The principle of gravimetry, analytical use. Volumetric analysis (volumetry): general procedure for determining, detecting the end point of a titration, titration curves, the calculation of the analyte from the titration data. Volumetric determination of types and their applications. 5. Electroanalytical methods based on voltage measurement: Equilibrium potentiometry - direct potentiometry. Indirect potentiometry. Principles, instrumentation, applications. 6. Electroanalytical methods based on current measurement. Voltammetry and polarography: principles, instrumentation, applications. Electrogravimetry and coulometry: implementing a constant current and constant potential. Conductometry. Principles, instrumentation, applications. 7. Overview of separation methods, principles and their analytical applications: Planar chromatography: thin layer chromatography (TLC + HPTLC), on paper, analytical applications. 8. Column chromatography - a general principle, the distribution of chromium. methods, instrumentation, qualitative analysis, quantitative analysis, chromatogram, analytical use. Characteristic differences in the experimental arrangement and implementation of gas and liquid chromatography. Using chromatographic methods. 9. Extraction - different types of extractions, the balance in the system, the extraction efficiency of the design, analytical applications: LLE, SFE, SPE, SPME. Trends in extraction methods. 10. Electrophoresis - principles, instrumentation, analytical applications: IEF, CE, isotachophoresis. Membrane separation: ultrafiltration, dialysis, reverse osmosis, electrodialysis. Mass spectrometry - principles of methods, instrumentation, mass spectrum, analytical use. 11. Overview of optical methods. Optical spectral apparatus. Refractometry, interferometry, polarimetry, nephelometry and turbidimetry: principles, instrumentation and analytical applications. 12. Atomic emission spectral analysis. Flame photometry. Atomic absorption spectrometry. UV / Vis spectroscopy, LED analysis. Principle of the method, instrumentation, analytical use. 13. Infrared spectroscopy. Raman spectrum - the principle of the method, analytical recovery. 14. X-ray fluorescence spectrometry - principles, instrumentation, analytical use. Radiochemical methods.

Conditions for subject completion

Part-time form (validity from: 1960/1961 Summer semester)
Task nameType of taskMax. number of points
(act. for subtasks)
Min. number of points
Exercises evaluation and Examination Credit and Examination 100 (100) 51
        Exercises evaluation Credit 30 (30) 0
                Project Project 20  0
                Written exam Written test 10  0
        Examination Examination 70 (70) 0
                Written examination Written examination 40  0
                Oral Oral examination 30  0
Mandatory attendence parzicipation:

Show history

Occurrence in study plans

Academic yearProgrammeField of studySpec.ZaměřeníFormStudy language Tut. centreYearWSType of duty
2009/2010 (B2102) Mineral Raw Materials (3904R005) Environmental Engineering P Czech Ostrava 1 Compulsory study plan
2009/2010 (B2102) Mineral Raw Materials (3904R005) Environmental Engineering K Czech Ostrava 1 Compulsory study plan
2008/2009 (B2102) Mineral Raw Materials (3904R005) Environmental Engineering K Czech Ostrava 1 Compulsory study plan
2008/2009 (B2102) Mineral Raw Materials (3904R005) Environmental Engineering P Czech Ostrava 1 Compulsory study plan
2007/2008 (B2102) Mineral Raw Materials (3904R005) Environmental Engineering K Czech Ostrava 1 Compulsory study plan

Occurrence in special blocks

Block nameAcademic yearForm of studyStudy language YearWSType of blockBlock owner