546-0354/05 – Instrumental Methods of Analysis (IMA)
| Gurantor department | Department of Environmental Engineering | Credits | 8 |
| Subject guarantor | doc. Mgr. Eva Pertile, Ph.D. | Subject version guarantor | doc. Mgr. Eva Pertile, Ph.D. |
| Study level | undergraduate or graduate | Requirement | Compulsory |
| Year | 1 | Semester | summer |
| | Study language | Czech |
| Year of introduction | 2006/2007 | Year of cancellation | 2009/2010 |
| Intended for the faculties | HGF | Intended for study types | Bachelor |
Subject aims expressed by acquired skills and competences
Obtaining basic knowledge not only about environmental sampling in relation to its share in the overall uncertainty of environmental analyzes but also in instrumental analytical methods. This will increase student readiness to solve analytic problems by selected instrumental methods.
Obtaining the competence to work in an analytical laboratory with basic instrumental equipment, the ability to present and evaluate the results, to learn the correct principles of working with the sample.
Teaching methods
Lectures
Experimental work in labs
Terrain work
Other activities
Summary
The lecture provides information about selected modern instrumental methods of research and monitoring of the environment, its use and application. During the lectures, students will get acquainted with the principles of analytical methods that are commonly used in control and development analytical laboratories.
Compulsory literature:
Recommended literature:
Way of continuous check of knowledge in the course of semester
Each laboratory exercise starts with a 5-minute 6-question test, to prove that the student read the manual (instructions to the laboratory exercise) and is able to work safely and purposefully. Three questions in the test need to be answered correctly to be allowed to work. If not the test is classified “0 points”. Each laboratory exercise is classified after delivering the final report or not classified if the report is not delivered or delivered late.
E-learning
Other requirements
Prerequisities
Subject has no prerequisities.
Co-requisities
Subject has no co-requisities.
Subject syllabus:
1. Defining the problem. Basic concepts. Processes of obtaining information. Types of interactions. Chemical equilibrium in analytical chemistry. Appraisal reactions.
2. Process of analysis. Sampling and sample treatment before analysis, separation and concentration steps. Evaluation of analytical results and procedures and expression analysis results. Proposal for action arising from the data obtained. Principles of good laboratory practice.
3. Trends in analytical chemistry - new instrumental procedures, including mobile analytics.
4. Quantitative chemical analysis. The principle of gravimetry, analytical use. Volumetric analysis (volumetry): general procedure for determining, detecting the end point of a titration, titration curves, the calculation of the analyte from the titration data. Volumetric determination of types and their applications.
5. Electroanalytical methods based on voltage measurement: Equilibrium potentiometry - direct potentiometry. Indirect potentiometry. Principles, instrumentation, applications.
6. Electroanalytical methods based on current measurement. Voltammetry and polarography: principles, instrumentation, applications. Electrogravimetry and coulometry: implementing a constant current and constant potential. Conductometry. Principles, instrumentation, applications.
7. Overview of separation methods, principles and their analytical applications: Planar chromatography: thin layer chromatography (TLC + HPTLC), on paper, analytical applications.
8. Column chromatography - a general principle, the distribution of chromium. methods, instrumentation, qualitative analysis, quantitative analysis, chromatogram, analytical use. Characteristic differences in the experimental arrangement and implementation of gas and liquid chromatography. Using chromatographic methods.
9. Extraction - different types of extractions, the balance in the system, the extraction efficiency of the design, analytical applications: LLE, SFE, SPE, SPME. Trends in extraction methods.
10. Electrophoresis - principles, instrumentation, analytical applications: IEF, CE, isotachophoresis. Membrane separation: ultrafiltration, dialysis, reverse osmosis, electrodialysis. Mass spectrometry - principles of methods, instrumentation, mass spectrum, analytical use.
11. Overview of optical methods. Optical spectral apparatus. Refractometry, interferometry, polarimetry, nephelometry and turbidimetry: principles, instrumentation and analytical applications.
12. Atomic emission spectral analysis. Flame photometry. Atomic absorption spectrometry. UV / Vis spectroscopy, LED analysis. Principle of the method, instrumentation, analytical use.
13. Infrared spectroscopy. Raman spectrum - the principle of the method, analytical recovery.
14. X-ray fluorescence spectrometry - principles, instrumentation, analytical use. Radiochemical methods.
Conditions for subject completion
Occurrence in study plans
Occurrence in special blocks
Assessment of instruction
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