546-0354/07 – Instrumental Methods of Analysis (IMA)

Gurantor departmentDepartment of Environmental EngineeringCredits5
Subject guarantordoc. Mgr. Eva Pertile, Ph.D.Subject version guarantordoc. Mgr. Eva Pertile, Ph.D.
Study levelundergraduate or graduateRequirementCompulsory
Year3Semesterwinter
Study languageCzech
Year of introduction2016/2017Year of cancellation2023/2024
Intended for the facultiesHGFIntended for study typesBachelor
Instruction secured by
LoginNameTuitorTeacher giving lectures
PER34 doc. Mgr. Eva Pertile, Ph.D.
SYR0013 Ing. Lucie Syrová
VAS0028 Ing. Michal Vaštyl, Ph.D.
Extent of instruction for forms of study
Form of studyWay of compl.Extent
Full-time Credit and Examination 1+3
Part-time Credit and Examination 3+9

Subject aims expressed by acquired skills and competences

Obtaining basic knowledge not only about environmental sampling in relation to its share in the overall uncertainty of environmental analyzes but also in instrumental analytical methods. This will increase student readiness to solve analytic problems by selected instrumental methods. Obtaining the competence to work in an analytical laboratory with basic instrumental equipment, the ability to present and evaluate the results, to learn the correct principles of working with the sample.

Teaching methods

Lectures
Experimental work in labs
Terrain work
Other activities

Summary

The lecture provides information about selected modern instrumental methods of research and monitoring of the environment, its use and application. During the lectures, students will get acquainted with the principles of analytical methods that are commonly used in control and development analytical laboratories.

Compulsory literature:

CHANG R.: Chemistry. 10th Edition. McGraw-Hill: New York, 2010. 1170 p. Dostupné z: https://archive.org/details/Chemistry_10th_Edition_Raymond_Chang PERTILE, E. Instrumental Methods of Analysis. Ostrava: Vysoká škola báňská - Technická univerzita Ostrava, 2017. ISBN 978-80-248-4124-3

Recommended literature:

ROUESSAC Francis and ROUESSAC, Annick: Chemical Analysis Modern Instrumentation Methods and Techniques. March 2007. 600 pp. ISBN: 978-0-470-85903-2 NOLLET M., L., Chromatographic Analysis of the Environment, Chromatographic Science Series, Volume 93, 1297 s. 2006, ISBN 0-8247-2629-4

Way of continuous check of knowledge in the course of semester

Student knowledge is verified in written form, emphasis is given to understanding the subject and applying theoretical knowledge to solving practical problems. The evaluation also includes collective evaluation and self-assessment. An active assessment of the students is also included in the class. Each lab starts with a 5-minute six-question test, which the student demonstrates that he reads the tutorials and is able to work safely and purposefully.

E-learning

Other requirements

Active participation in the exercises, demonstrated the successful processing of subtasks, entered continuously in the semester.

Prerequisities

Subject has no prerequisities.

Co-requisities

Subject has no co-requisities.

Subject syllabus:

1. Definition of the issue. Information-gathering process. The analysis process. Evaluation of analytical results and practices, expression analysis results. Statistical results. Draft action arising from the data obtained. Principles of Good Laboratory Practice. Basics of chemometrics. 2. Sampling, the general principle and sources of the errors. Standard operating procedures for sampling; QA / QC system. Basic parameters sampling of abiotic and biotic matrices. 3. Treatment of the sample before analysis. Nondestructive methods. Destructive methods. Separation and concentration steps. 4. Qualitative analysis: classical system of dividing cations. Evidence heteroatoms in organic compounds. 5. Overview of the basic analytical methods and their general properties: methods based on weighing, measuring volume. The principles of individual methods; instrumentation; application in the analysis of environmental components; advantages/disadvantages. 6. Electrochemical methods based on the voltage measurement. Electrochemical methods based on the current measurement. The principles of individual methods; instrumentation; application in the analysis of environmental components; advantages/disadvantages. 7. Summary separation methods: Planar chromatography: TLC + HPTLC, PC, advantages/disadvantages. 8. Column chromatography: GC + HPLC. New trends: UHPLC, GC / MS, HPLC / MS. The principles of individual methods; instrumentation; application in the analysis of environmental components; advantages/disadvantages. 9. Electrophoresis. Mass spectrometry. The principles of individual methods; instrumentation; application in the analysis of environmental components; advantages/disadvantages. 10. Overview of the optical methods. Optical spectral device. Refractometry, interferometry, polarimetry and turbidimetry: Principles of the individual methods; instrumentation; application in the analysis of the environmental components; advantages/disadvantages. 11. Atomic emission spectral analysis. Flame photometry. Atomic absorption spectrometry. UV / Vis spectrometry. The principles of individual methods; instrumentation; application in the analysis of environmental components; advantages/disadvantages. 12. Luminescence analysis. Infrared spectrometry. Raman spectra; X-ray fluorescence spectrometry. Radiochemical methods. Understanding the various methods, instrumentation, application in the analysis of environmental components; advantages/disadvantages. 13. New trends in instrumental methods with applications in biotechnology.

Conditions for subject completion

Part-time form (validity from: 2016/2017 Winter semester, validity until: 2023/2024 Summer semester)
Task nameType of taskMax. number of points
(act. for subtasks)
Min. number of pointsMax. počet pokusů
Credit and Examination Credit and Examination 100 (100) 51
        Credit Credit 33 (33) 17
                Protokoly Laboratory work 15  8
                Výpočtový protokol Laboratory work 10  5
                Zápočtový test Written test 8  4
        Examination Examination 67  34 3
Mandatory attendence participation: 3 hours of lectures + 9 hours of exercises

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Conditions for subject completion and attendance at the exercises within ISP: As part of the ISP, the student is obliged to participate in individual consultations, which will be conducted in the form of a discussion on the topics studied. Exercise: The student submits a calculation report.

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Occurrence in study plans

Academic yearProgrammeBranch/spec.Spec.ZaměřeníFormStudy language Tut. centreYearWSType of duty
2023/2024 (B2102) Mineral Raw Materials (3904R005) Environmental Engineering P Czech Ostrava 3 Compulsory study plan
2022/2023 (B2102) Mineral Raw Materials (3904R005) Environmental Engineering P Czech Ostrava 3 Compulsory study plan
2021/2022 (B2102) Mineral Raw Materials (3904R005) Environmental Engineering P Czech Ostrava 3 Compulsory study plan
2020/2021 (B2102) Mineral Raw Materials (3904R005) Environmental Engineering P Czech Ostrava 3 Compulsory study plan
2020/2021 (B2102) Mineral Raw Materials (3904R005) Environmental Engineering K Czech Ostrava 3 Compulsory study plan
2019/2020 (B2102) Mineral Raw Materials (3904R005) Environmental Engineering P Czech Ostrava 3 Compulsory study plan
2019/2020 (B2102) Mineral Raw Materials (3904R005) Environmental Engineering K Czech Ostrava 3 Compulsory study plan
2018/2019 (B2102) Mineral Raw Materials (3904R005) Environmental Engineering P Czech Ostrava 3 Compulsory study plan
2018/2019 (B2102) Mineral Raw Materials (3904R005) Environmental Engineering K Czech Ostrava 3 Compulsory study plan
2017/2018 (B2102) Mineral Raw Materials (3904R005) Environmental Engineering P Czech Ostrava 3 Compulsory study plan
2017/2018 (B2102) Mineral Raw Materials (3904R005) Environmental Engineering K Czech Ostrava 3 Compulsory study plan
2016/2017 (B2102) Mineral Raw Materials (3904R005) Environmental Engineering P Czech Ostrava 3 Compulsory study plan
2016/2017 (B2102) Mineral Raw Materials (3904R005) Environmental Engineering K Czech Ostrava 3 Compulsory study plan

Occurrence in special blocks

Block nameAcademic yearForm of studyStudy language YearWSType of blockBlock owner

Assessment of instruction



2022/2023 Winter
2018/2019 Winter
2017/2018 Winter