546-0354/08 – Instrumental Methods of Analysis (IMA)
| Gurantor department | Department of Environmental Engineering | Credits | 5 |
| Subject guarantor | doc. Mgr. Eva Pertile, Ph.D. | Subject version guarantor | doc. Mgr. Eva Pertile, Ph.D. |
| Study level | undergraduate or graduate | Requirement | Compulsory |
| Year | 3 | Semester | winter |
| | Study language | Czech |
| Year of introduction | 2022/2023 | Year of cancellation | |
| Intended for the faculties | HGF | Intended for study types | Bachelor |
Subject aims expressed by acquired skills and competences
Obtaining basic knowledge not only about environmental sampling in relation to its share in the overall uncertainty of environmental analyzes but also in instrumental analytical methods. This will increase student readiness to solve analytic problems by selected instrumental methods.
Obtaining the competence to work in an analytical laboratory with basic instrumental equipment, the ability to present and evaluate the results, to learn the correct principles of working with the sample.
Teaching methods
Lectures
Experimental work in labs
Terrain work
Other activities
Summary
The lecture provides information about selected modern instrumental methods of research and monitoring of the environment, its use and application. During the lectures, students will get acquainted with the principles of analytical methods that are commonly used in control and development analytical laboratories.
Compulsory literature:
Recommended literature:
Way of continuous check of knowledge in the course of semester
Student knowledge is verified in written form, emphasis is given to understanding the subject and applying theoretical knowledge to solving practical problems. The evaluation also includes collective evaluation and self-assessment. An active assessment of the students is also included in the class. The course is completed by a credit and a combined exam (written and oral).
E-learning
Other requirements
Active participation in the exercises, demonstrated the successful processing of subtasks, entered continuously in the semester.
Prerequisities
Subject has no prerequisities.
Co-requisities
Subject has no co-requisities.
Subject syllabus:
1. Defining the issue. Information retrieval processes. Evaluation of analytical results and procedures, expression of analysis results. Statistical processing of results. Proposal of activities resulting from the obtained data. GLP principles.
2. Sampling, general principles of the source of errors. Standard Operating Procedures for Sampling; QA / QC system.
3. Sample preparation before analysis. Non-destructive methods. Destructive methods. Separation and concentration steps.
4. Basics of qualitative analysis
5. Overview of basic analytical methods and their general properties: methods based on weighing, on volume measurement. Principles of individual methods; instrumentation; applications in the analysis of environmental components; Advantages, disadvantages.
6. Electroanalytical methods based on voltage and current measurements. Applications in the analysis of environmental components; Advantages, disadvantages.
7. Overview of separation methods: application in the analysis of environmental components; Advantages, disadvantages.
8. Column chromatography: GC + HPLC. New trends: UHPLC, GC / MS, HPLC / MS. Applications in the analysis of environmental components; Advantages, disadvantages.
9. Electrophoresis. Mass spectrometry. Applications in the analysis of environmental components; Advantages, disadvantages.
10. Overview of optical methods. Optical spectral instruments. Applications in the analysis of environmental components; Advantages, disadvantages.
11. Emission atomic spectral analysis. Flame photometry. Atomic absorption spectrometry. UV / Vis spectrometry. Applications in the analysis of environmental components; Advantages, disadvantages.
12. IR spectrometry. Raman spectra; X-ray fluorescence spectrometry. Applications in the analysis of environmental components; Advantages, disadvantages.
13. New trends in instrumental methods with application in the analysis of environmental components
Conditions for subject completion
Occurrence in study plans
Occurrence in special blocks
Assessment of instruction
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