546-0359/01 – Basics of Biotechnology (ZB)

Gurantor departmentDepartment of Environmental EngineeringCredits6
Subject guarantordoc. Mgr. Hana Vojtková, Ph.D.Subject version guarantorprof. Ing. Peter Fečko, CSc.
Study levelundergraduate or graduateRequirementCompulsory
Study languageCzech
Year of introduction2006/2007Year of cancellation2008/2009
Intended for the facultiesHGFIntended for study typesBachelor
Instruction secured by
LoginNameTuitorTeacher giving lectures
FEC40 prof. Ing. Peter Fečko, CSc.
PEC06 Ing. Iva Janáková, Ph.D.
Extent of instruction for forms of study
Form of studyWay of compl.Extent
Full-time Credit and Examination 3+2

Subject aims expressed by acquired skills and competences

Target competence on the basis of new knowledge and its application in problem solving, observation, experiments and communication: - to characterize differences in the structure of cells and live organisms, to be well informed about the natural systems and to understand phylogenetic relations of the individual groups, to learn about and to term characteristic organisms of the individual taxons and to determine their significance - to apply the gained knowledge in dealing with practical tasks in seminars, to consider the consequences of a phenomenon from the environmental, health as well as economic points of view and to justify the significance of new biological information for the society - ability of independent solution of set tasks, to observe and objectively record experiment results, to document observation in the form of diagrams and charts with legends, to adhere to safety regulations when working with biological material and technical instruments - ability to work with technical texts, to master specialist terminology, to synthetically evaluate data acquired in dealing with tasks in seminars, to defend an opinion of a biological problem solution in a discussion

Teaching methods

Experimental work in labs


The course focuses on clarifying the role of microorganisms in biotechnologies in wastewater treatment. Students gain information on the morphology and physiology of non-cellular organisms, basic groups of microorganisms, their role in the nature and mutual relations of microbial populations with an overview of fundamental pathogenic as well as non-pathogenic strains of microorganisms. Some lectures are dedicated to a practical utilization of microorganisms in industrial biotechnologies and their role in the formation and protection of the environment.

Compulsory literature:

GLAZER, Alexander N. a Hiroshi NIKAIDO. Microbial biotechnology : fundamentals of applied mikrobiology. 2nd ed. New York: Cambridge University Press, 2007. xvii, 554 s. ISBN 978-0-521-84210-5 MIMS, Cedric A. (ed.) Medical microbiology. 3rd ed. Edinburgh: Elsevier, 2004. ix, 660 s. ISBN 0-323-03575-2

Recommended literature:

MOAT, Albert G., John W. FOSTER a Michael P. SPECTOR. Microbial Physiology. New York: Wiley-Liss, 2002. 714 s. ISBN 978-0-471-39483-9

Way of continuous check of knowledge in the course of semester


Other requirements


Subject has no prerequisities.


Subject has no co-requisities.

Subject syllabus:

Přednášky: 1. Úvod. Mikrobiológia ako veda. Rozdelenie mikroorganizmov. Rozdelenie bakterií podľa fyziologických vlastností. 2. Bunka - zloženie mikrobiálnych buniek. Baktérie - morfológia, štruktúra bakteriálnej bunky rozmnožovanie baktérií rozdelenie baktérií 3. Kvasinky - cytológia kvasiniek rozmnožovanie kvasiniek rozdelenie kvasiniek 4. Pliesne - základné charakteristiky rozdelenie pliesní Riasy - základné charakteristiky rozdelenie rias 5. Základné rysy metabolizmu mikroorganizmov Vplyv vonkajšieho prostredia na mikroorganizmy 6. Dynamika rastu a množenia mikroorganizmov 7. Charakteristika baktérií rodu Thiobacillus ferrooxidans 8. Mechanizmus bakteriálnej oxidácie sulfidov baktériami Thiobacillus ferrooxidans (priama a nepriama). 9. Faktory ovplyvňujúce činnosť baktérií T.F. Kultivačné média pre T.F. lO.Technika postupu bakteriálneho lúhovania. Technika lúhovania rúd. ll.Bakteriálne desulfurizácia uhlia. l2.Silikátové baktérie - ich účinok na silikáty - degradačné mechanizmy - využitie v praxi l3.Bioakumulácia a dočisťovanie vôd mikroorganizmami. l4.Ropné baktérie a dekontaminácia pôd. l5.Nové smery a technológie pri aplikácii mikroorganizmov v úpravníctve. Cvičení: l. Úvod. Základné podmienky pre prácu v mikrobiálnom laboratóriu. 2. Príprava živných pôd pre mikroorganizmy (všetky druhy pevných médií). Základné práce s mikrobiálnou kľučkou a s médiami. 3. Príprava mikrobiálnych preparátov. Grammove skúšky. 4. Identifikácia a sledovanie morfológie baktérií. 5. Identifikácia a sledovanie morfológie kvasiniek. 6. Stanovenie mikrobiálneho znečistenia vôd. 7. Príprava živných médií a množenie baktérií Thiobacillus ferrooxidans. 8. Príprava preparátov baktérií Thiobacillus ferrooxidans. 9. Počítanie baktérií - mikroskopická a MPN - metóda. lO.Odsírovanie hnedého uhlia baktériami T.F. ll.Aplikácia baktérii pri spracovaní odpadov z hutníctva a baníctva. l2.Vyhodnotenie testov z predchádzajúceho týždňa, stanovenie Fe2+ a počtu baktérií po týždni lúhovania. l3.Vyhodnotenie testov po 2.týždňoch lúhovania. l4.Vyhodnotenie testov po 3. týždňoch lúhovania a zhodnotenie aplikácie daného spôsobu úpravy. l5.Udelenie zápočtov.

Conditions for subject completion

Full-time form (validity from: 1960/1961 Summer semester)
Task nameType of taskMax. number of points
(act. for subtasks)
Min. number of points
Exercises evaluation and Examination Credit and Examination 100 (100) 51
        Exercises evaluation Credit 33 (33) 0
                Other task type Other task type 33  0
        Examination Examination 67 (67) 0
                Written examination Written examination 30  0
                Oral Oral examination 37  0
Mandatory attendence parzicipation:

Show history

Occurrence in study plans

Academic yearProgrammeField of studySpec.ZaměřeníFormStudy language Tut. centreYearWSType of duty
2008/2009 (B2102) Mineral Raw Materials (3904R022) Waste Treatment and Disposal P Czech Ostrava 3 Compulsory study plan
2007/2008 (B2102) Mineral Raw Materials (3904R022) Waste Treatment and Disposal P Czech Ostrava 3 Compulsory study plan
2006/2007 (B2102) Mineral Raw Materials (3904R022) Waste Treatment and Disposal P Czech Ostrava 3 Compulsory study plan

Occurrence in special blocks

Block nameAcademic yearForm of studyStudy language YearWSType of blockBlock owner