546-0371/01 – Biochemistry (BIO)
| Gurantor department | Department of Environmental Engineering | Credits | 3 |
| Subject guarantor | prof. Ing. Daniela Plachá, Ph.D. | Subject version guarantor | prof. Ing. Daniela Plachá, Ph.D. |
| Study level | undergraduate or graduate | Requirement | Choice-compulsory |
| Year | 2 | Semester | winter |
| | Study language | Czech |
| Year of introduction | 2006/2007 | Year of cancellation | 2017/2018 |
| Intended for the faculties | HGF | Intended for study types | Bachelor |
Subject aims expressed by acquired skills and competences
The aim of Biochemistry lectures is introduction of students to Biochemistry and Biotechnology basic knowledge.
Teaching methods
Lectures
Individual consultations
Summary
The biochemistry lectures are aimed to student introduction to basic knowledge of biochemistry showing up chemical structure, properties and reactivity of compounds that form base of living organisms. Further, it is aimed to principles of metabolisms and transformation of energy. The basic lectures of biochemistry are completed with overwiev of applications in practice.
Compulsory literature:
CH. K. Mathews, K. E. van Holde, K. G. Ahern. Biochemistry. Prentice Hall; 3 edition 1999.
D. L. Nelson, M. M. Cox. Lehninger Principles of Biochemistry, Fourth Edition, W. H. Freeman, 2008.
Recommended literature:
Nelson, D.L., Cox, M.M. Lehninger Principles of Biochemistry. W. H. Freeman, 2012.
Campbell M., Farrell S. Biochemistry. 2007.
Way of continuous check of knowledge in the course of semester
E-learning
Other requirements
Student will prepare the seminary paper on a defined topic.
Prerequisities
Subject has no prerequisities.
Co-requisities
Subject has no co-requisities.
Subject syllabus:
1. Introduction. Description of biochemistry and sphere of research. Composition and organization of living matter. Basic unit of living organisms, biopolymers, principles of metabolisms and energy transformation.
2. Peptides and proteins. Amino acides, structure, chemical and physical properties, chirality, importance of amino acides, isolation and analysis of amino acides.
3. Peptides and proteins. Structure, sequences and their determination, biochemical significant peptides. Strukture of proteins, conformation and their changes, allostery.
Classification of proteins. Isolation of proteins. Methods of protein research.
4. Nucleic acids. Nukleotides. Composition and structure of DNA and RNA,
properties, methods of research.
5. Saccharides. Properties, classification of monosaccharides. Monosaccharides and their derivatives, di-, oligo- and polysaccharides. Structures, chirality
and function in living organisms. Glycosides. Glycoproteins and proteoglycans. Analysis of
saccharides.
6. Lipids. Compositions, properties, function in organisms. Neutral
lipids, waxes, polar lipids. Steroides. Structure and significancy of biomembranes.
7. Enzymes. Structure, confirmation, properties, classification, nomenclature.
Occurence of enzymes. Activity. Coenzymes. Applied enzymology.
8. Mechanisms and kinetics of enzymatic reactions. Factors influencing of enzymatic reactions. Inhibitors. Other biocatalyzators.
9. Metabolisms and energy transformations in living systems. Catabolism and anabolism. Biochemical reactions, bioenergy.
10. Respiratory chain and a oxidative fosforylation. Photosynthesis. Transfer of
electrons and protons in respiratory chain. Biological oxidation. Krebs cycle. Glyoxalate cycle.
11. Metabolisms of saccharides and lipids. Glykolysis. Fermentation.
Metabolisms of lipids. Metabolisms of glycerol. Biosynthesis of lipids.
12. Metabolisms of proteins, nucleic acids, and other nitroegen-containig compounds. Degradation of amino acides. Biosynthesis amino acides.
13. Biochemistry of microorganisms. Dividing according of carbon and energy source.
Microorganisms in environment. Influence of the environment on living organisms. Cycles of biogenic elements in nature. Biodegradation, biosorption.
14.-15. Application of biochemistry in technologies. Food and alimentary, biotechnology,
formation of biogas, treatment of minerals, waste disposal.
Conditions for subject completion
Occurrence in study plans
Occurrence in special blocks
Assessment of instruction