546-0396/01 – Instrumental Methods of Analysis II (IMA II)
Gurantor department | Department of Environmental Engineering | Credits | 8 |
Subject guarantor | doc. Mgr. Eva Pertile, Ph.D. | Subject version guarantor | doc. Mgr. Eva Pertile, Ph.D. |
Study level | undergraduate or graduate | Requirement | Compulsory |
Year | 3 | Semester | winter |
| | Study language | Czech |
Year of introduction | 2006/2007 | Year of cancellation | 2019/2020 |
Intended for the faculties | HGF | Intended for study types | Bachelor |
Subject aims expressed by acquired skills and competences
Knowledge acquired after the completion of the course: Acquiring basic knowledge of instrumental analytical methods and enhancing students' readiness to solve analytic problems by selected instrumental methods.Skills demonstrated at the end of the course: The student will gain the competence to work in an analytical laboratory with basic instrumental equipment.
Teaching methods
Lectures
Experimental work in labs
Terrain work
Other activities
Summary
The course is focused on broadening and deepening the knowledge of modern instrumental laboratory techniques, including gaining practical skills. The aim of the course is to provide students with a solid and sufficiently broad theoretical and methodological basis for methods of instrumental analysis, both inorganic and organic. The main focus of the lessons is the interpretation of the theoretical foundations of instrumental methods of analysis. Practical exercises emphasize students' independent approach to solving analytical problems of selected instrumental methods.
Compulsory literature:
Recommended literature:
Way of continuous check of knowledge in the course of semester
Student knowledge is verified in written form, emphasis is given to understanding the subject and applying theoretical knowledge to solving practical problems. The evaluation also includes collective evaluation and self-assessment. An active assessment of the students is also included in the class.
E-learning
Other requirements
Active participation in the lectures and the seminars, demonstration of successful processing subtasks, entered continuously in the semester.
Prerequisities
Subject has no prerequisities.
Co-requisities
Subject has no co-requisities.
Subject syllabus:
1. Definition of issues. Basic concepts. Information retrieval processes. Analytical methods absolute and comparative. Calibration methods. Developmental trends in instrumental methods of environmental samples analysis.
2. Evaluation of the results of the analysis and the way of their expression with a focus on environmental analysis. Statistical evaluation of analytical results. Reference material, round test. Fundamentals of chemometric approaches and methods.
3. Testing the metrological properties of the results. Activity design resulting from the data obtained.
4. Electroanalytical methods: methods based on potential measurement (equilibrium potentiometry, potentiometric titration). Principles of individual methods; instrumentation; applications for environmental component analysis; Advantages, disadvantages.
5. Electroanalytical methods: current measurement methods. Voltammetry and polarography: Principles of individual methods; instrumentation; applications for environmental component analysis; Advantages, disadvantages.
6. Electrogravimetry and coulometry: a constant current and a constant potential. Conductometry. Principles of individual methods; instrumentation; applications for environmental component analysis; Advantages, disadvantages.
7. Optical analytical methods: theoretical basics. optical spectral devices. Methods without energy-to-radiation exchange (refractometry, polarimetry, nephelometry); Refractometry, Principles of individual methods; instrumentation; applications for environmental component analysis; Advantages, disadvantages.
8. Emission Atomic Spectral Analysis. Flame photometry. Atomic absorption spectrometry; UV / Vis spectrometry, Luminescence analysis. Fundamentals of theory, instrumentation, qualitative and quantitative aspects. Automatic analyzers with optical detection: FIA, CFA. Principles of individual methods; instrumentation; applications for environmental component analysis; Advantages, disadvantages.
9. Luminescence analysis. Infrared spectrometry. Raman spectra; X-ray fluorescence spectrometry. Radiochemical methods. Principles of individual methods, instrumentation, applications in environmental component analysis; Advantages, disadvantages.
10. Separation and concentration steps: theory. Separation methods: separation according to principle and according to experimental arrangement, efficiency of the separation process. Extraction. Instrumentation; applications for environmental component analysis; Advantages, disadvantages.
11. Membrane Separation. Mass spectrometry. Principles of individual methods; instrumentation; applications for environmental component analysis; Advantages, disadvantages.
12. Chromatographic methods. Planar Chromatography: Sorbent Thin Layer Chromatography, Fundamentals of Theory, Instrumentation, Qualitative and Quantitative Aspects, Applications.
13. Column chromatography: GC + HPLC. New Trends: UHPLC, GC / MS, HPLC / MS. Principles of individual methods; instrumentation; applications for environmental component analysis; Advantages, disadvantages.
14. Electromigration methods: theory, zonal and capillary electrophoresis, carrier electrophoresis and isotachophoresis. Principles of individual methods; instrumentation; applications for environmental component analysis; Advantages, disadvantages.
Conditions for subject completion
Occurrence in study plans
Occurrence in special blocks
Assessment of instruction