546-0396/02 – Instrumental Methods of Analysis II (IMA II)

Gurantor departmentDepartment of Environmental EngineeringCredits6
Subject guarantordoc. Mgr. Eva Pertile, Ph.D.Subject version guarantordoc. Mgr. Eva Pertile, Ph.D.
Study levelundergraduate or graduateRequirementCompulsory
Year2Semestersummer
Study languageCzech
Year of introduction2009/2010Year of cancellation2015/2016
Intended for the facultiesHGFIntended for study typesBachelor
Instruction secured by
LoginNameTuitorTeacher giving lectures
BOZ0008 Ing. Aleš Božoň
PER34 doc. Mgr. Eva Pertile, Ph.D.
Extent of instruction for forms of study
Form of studyWay of compl.Extent
Full-time Credit and Examination 1+4
Part-time Credit and Examination 12+6

Subject aims expressed by acquired skills and competences

Knowledge acquired after the completion of the course: Acquiring basic knowledge of instrumental analytical methods and enhancing students' readiness to solve analytic problems by selected instrumental methods.Skills demonstrated at the end of the course: The student will gain the competence to work in an analytical laboratory with basic instrumental equipment.

Teaching methods

Lectures
Experimental work in labs
Terrain work
Other activities

Summary

The course is focused on broadening and deepening the knowledge of modern instrumental laboratory techniques, including gaining practical skills. The aim of the course is to provide students with a solid and sufficiently broad theoretical and methodological basis for methods of instrumental analysis, both inorganic and organic. The main focus of the lessons is the interpretation of the theoretical foundations of instrumental methods of analysis. Practical exercises emphasize students' independent approach to solving analytical problems of selected instrumental methods.

Compulsory literature:

PERTILE, E. Instrumental Methods of Analysis. Ostrava: Vysoká škola báňská - Technická univerzita Ostrava, 2017. ISBN 978-80-248-4124-3. SIVASANKAR, B. Instrumental methods of analysis. New Delhi: Oxford University Press, 2012. ISBN 9780198073918. BRICKER, B., ed. Experiments in analytical chemistry. London: Auris Reference, c2012. ISBN 978-1-78154-179-1. CHANG R.: Chemistry. 10th Edition. McGraw-Hill: New York, 2010. 1170 p. Available from: https://archive.org/details/Chemistry_10th_Edition_Raymond_Chang.4-3.

Recommended literature:

LONG, J. H. A textbook of elementary analytical chemistry: qualitative and volumetric. Lexington, KY: [CreateSpace Independent Publishing Platform], 2014. ISBN 978-1-4927-1822-2. TISSUE, B. M. Basics of analytical chemistry and chemical equilibria. Hoboken: Wiley, c2013. ISBN 978-0-470-59208-3. CAROLI, S. and G. ZÁRAY, ed. Analytical techniques for clinical chemistry: methods and applications. Hoboken: Wiley, c2012. ISBN 978-0-470-44527-3. GUARDIA, M. de la a S. GARRIGUES MATEO, ed. Handbook of green analytical chemistry. Chichester: Wiley, 2012. ISBN 978-0-470-97201-4.

Way of continuous check of knowledge in the course of semester

Knowledge control is checked in each exercise - check tests.

E-learning

Other requirements

Active participation in lectures and seminars.

Prerequisities

Subject has no prerequisities.

Co-requisities

Subject has no co-requisities.

Subject syllabus:

1. Introduction to instrumental methods, their advantages, disadvantages, the distribution of instrumental methods, their qualitative and quantitative use. Defining the problem. Basic terms. Processes of obtaining information. Absolute and comparative analytical methods. Calibration methods. Developments in instrumental methods of analysis. 2. Evaluating results of analysis and their expression. Statistical evaluation of analytical results. Reference material, a circular test. Fundamentals of chemometric approaches and methods. Testing the metrological characteristics of results. 3. - 5. Electroanalytical methods: their classification and principles, cells, electrode potential, Nernst and Peterson equation. Methods based on measuring potential: Principles, instrumentation layout, uses, advantages and disadvantages. Electrodes and specific cells. Glass pH electrode, experimental approach to measure pH. Ion-selective electrodes. The choice of the electrode system. Nikolsky-Eisenman equation. Electrodes with additional membranes. Specific cell sets for potentiometric titration, evaluation of titration curves. Direct potentiometry, the method of calibration line, the method of standard additions, potentiometric titration, evaluation of titration curves, examples of use. Methods based on current measurement: Voltammetry and polarography: Fundamentals of polarografic measurements: principle, instrument layout, qualitative and quantitative analysis, use. Mercury drop electrode and its properties. Currents, maximum. Sample treatment for polarographic measurements. Types of titration curves, their interpretation, applications. DC, AC, pulse, differential, inverse; curves shapes, evaluation, and charging the electrolytic current apparatus, calibration methods, examples of use. Current trends in voltammetry. Amperometry, biamperometry - principles of methods, forms and evaluation of titration curves, examples of use. Electrogravimetry - electrolysis. Faraday's law, elimination of products on the cathode and anode, apparatus, examples of use. Coulometry: principles, coulometry at a constant potential, coulometry at a constant current, coulometric titration, evaluation, chronopotentiometry. Conductometry: mobility of ions, Kohlrausch law, specific and molar conductivity, direct conductometry, calibration, conductometric titration, shapes and evaluation of titration curves, examples of use. Principles, instrumentation, analytical applications, advantages and disadvantages. 6. - 9. Optical methods, interaction of matter and radiation, the classification of optical methods, principles, fundamentals of luminescence analysis, use of optical methods. Optical analytical methods: theoretical basics. Optical spectral apparatus. Non-spectroskopic methods - methods without enery changes between the substance and radiation, a) refractometry- refraction, method principle, apparatus, application examples, b) polarimetry - polarized light, optically active substance, the principle of the method, apparatus, examples of use. Spectroscopic methods: absorption and emission of radiation, the energy levels of atoms and molecules, degradation of polychromatic radiation by prism and grating, monochromators, interference filters. Emission methods - excitation, electron spectrum a) atomic emission spectroscopy - principles, equipment, registration of spectra, the qualitative and quantitative evaluation, usage examples, b) flame emission spectroscopy - principles, equipment, calibration methods, examples of use. Absorption methods - absorption of light by atoms and molecules: theory basics, instrumentation, qualitative and quantitative aspects, applications, analytical applications. Atomic absorption spectrometry: principle, flame and flameless atomization, apparatus, calibration methods, examples of use. Molecular absorption spectrometry: principle, transmittance, absorbance, Lambert-Beer law, apparatus, calibration methods, examples of use. Automatic analyzers with optical detection: FIA, CFA. Infrared spectroscopy: Principle, apparatus, instrumentation, evaluation of molecular absorption spectra, analytical use. Raman spectrum - the principle of the method, analytical recovery. Absorption of radio waves and microwaves. Nuclear magnetic resonance (NMR) and electron paramagnetic resonance (EPR). 10. Separation and concentration steps: theory. Separation methods: separation based on the principle and according to the experimental design, the effectiveness of separation process. Extraction. 11. Membrane separation. Mass spectrometry. Basic theory, instrumentation, qualitative and quantitative aspects of application. 12. Chromatographic methods. Planar Chromatography: Thin layer sorbent chromatography, theory basics, instrumentation, qualitative and quantitative aspects of application. 13. Column chromatography: Liquid chromatography. Gas chromatography. Theory, basic instrumentation, qualitative and quantitative characteristics, analytical use. 14. Electromigration methods: theory, zonal and capillary electrophoresis, electrophoresis on medium and isotachophoresis. Basics of instrumentation, qualitative and quantitative characteristics, analytical use. Exercise syllabus Lectures and exercises are conceived as a one-block course for students of combined from of study.

Conditions for subject completion

Full-time form (validity from: 2009/2010 Summer semester, validity until: 2011/2012 Summer semester)
Task nameType of taskMax. number of points
(act. for subtasks)
Min. number of pointsMax. počet pokusů
Exercises evaluation and Examination Credit and Examination 100 (100) 51 2
        Exercises evaluation Credit 45 (45) 23 1
                Lab1 - 6 Laboratory work 24  0
                Opakování z předmětu IMA I Written test 8  4 1
                Závěrečný zápočtový test Written test 8  4 1
                Protokol z terénu Other task type 5  0
        Examination Examination 55 (55) 23 3
                Písemná zkouška Written examination 25  13
                Ústní zkouška Oral examination 30  10
Mandatory attendence participation:

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Conditions for subject completion and attendance at the exercises within ISP:

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Occurrence in study plans

Academic yearProgrammeBranch/spec.Spec.ZaměřeníFormStudy language Tut. centreYearWSType of duty
2014/2015 (B2102) Mineral Raw Materials (3904R022) Waste Treatment and Disposal P Czech Ostrava 2 Compulsory study plan
2013/2014 (B2102) Mineral Raw Materials (3904R022) Waste Treatment and Disposal P Czech Ostrava 2 Compulsory study plan
2012/2013 (B2102) Mineral Raw Materials (3904R022) Waste Treatment and Disposal P Czech Ostrava 2 Compulsory study plan
2011/2012 (B2102) Mineral Raw Materials (3904R022) Waste Treatment and Disposal P Czech Ostrava 2 Compulsory study plan
2010/2011 (B2102) Mineral Raw Materials (3904R022) Waste Treatment and Disposal P Czech Ostrava 2 Compulsory study plan
2009/2010 (B2102) Mineral Raw Materials (3904R022) Waste Treatment and Disposal P Czech Ostrava 2 Compulsory study plan

Occurrence in special blocks

Block nameAcademic yearForm of studyStudy language YearWSType of blockBlock owner

Assessment of instruction

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