546-0807/02 – Modern Biotechnology Methods (MMB)

Gurantor departmentDepartment of Environmental EngineeringCredits3
Subject guarantordoc. Mgr. Hana Vojtková, Ph.D.Subject version guarantordoc. Mgr. Hana Vojtková, Ph.D.
Study levelundergraduate or graduateRequirementCompulsory
Study languageCzech
Year of introduction2015/2016Year of cancellation2019/2020
Intended for the facultiesHGFIntended for study typesFollow-up Master
Instruction secured by
LoginNameTuitorTeacher giving lectures
VOJ195 doc. Mgr. Hana Vojtková, Ph.D.
Extent of instruction for forms of study
Form of studyWay of compl.Extent
Full-time Graded credit 2+0

Subject aims expressed by acquired skills and competences

Target competence on the basis of new knowledge and its application in communication: - to perceive biotechnology as a multidisciplinary scientific discipline, to learn about the current directions of its development focusing on state-of-the-art methods of genetic engineering; to characterize differences in the organization of prokaryotic and eukaryotic cell genomes, to understand the meaning of modern methods based on DNA technologies on the grounds of identifying structural changes in cell genotypes - to consider the practical consequences of a phenomenon from the environmental, health as well as economic points of view and to justify the significance of new information on molecular genetics and genetic engineering for the society - to understand the principles of basic methods of genetic engineering leading to modifications of the existing microorganisms and thus to a synthesis of new products - ability to work with technical texts, to master specialist terminology, to be well informed about the contents of new genetic disciplines, to get to know the topical principles of world-wide databases and collections of microorganisms

Teaching methods



The content of the subject builds on the acquired knowledge of biotechnology and expands the fundamentals in biological science. The own profile of the subject sees the status of modern biotechnology as a multidisciplinary discipline on the boundary of technical and biological sciences. The objective of the subject is to expand the knowledge of biotechnology with new approaches in biotechnological practice which are nowadays based on topical knowledge of genomics, proteomics and other disciplines of genetic engineering. Students are acquainted with cellular processes on a molecular level and with their mutual interaction at an origin of important biological macromolecules participating in genetic properties of organisms. The content of lectures is theoretical basics of common and more specialized methodical procedures applied in molecular-biological research, which complement modern technologies of genetic engineering asserted especially in the construction of transgenic microorganisms for specific technological applications in industry and environmental protection.

Compulsory literature:

MITCHELSON, Keith R. (ed.). New high throughput technologies for DNA sequencing and genomics. Amsterdam: Elsevier, 2007. ISBN 978-0-444-52223-8 RÉDEI, George P.. Encyclopedia of Genetics, Genomics, Proteomics, and Informatics. 3rd Edition. Springer, 2008. ISBN 978-1-4020-6753-2

Recommended literature:

BRANDEN, Carl a John TOOZE. Introduction to protein structure. 2nd ed. New York: Garland Publishing, 1998. xiv, 410 s. ISBN 0-8153-2304-2 NYRÉN, P. The History of Pyrosequencing. Methods Mol Biology, 2007, roč. 373, s. 1–14. ISSN: 1064-3745 KING, Robert C., William D. STANSFIELD William D. a Pamela K. MULLIGAN. A Dictionary of Genetics. Oxford University Press, 2006. ISBN 978-0-19-514325-6

Way of continuous check of knowledge in the course of semester

Průběžná kontrola studia je vedena formou diskuse k přednášené problematice.


Other requirements

- elaboration of homeworks


Subject has no prerequisities.


Subject has no co-requisities.

Subject syllabus:

1. Introduction to the genetic engineering 2. Basics of modern biotechnology 3. Basics of cytogenetics 4. Mutation 5. Methods for testing of mutagens and carcinogens 6. Molecular genetics 7. Genetic code and gene expression 8. Introduction to the proteomics 9. Basics of genetic engineering 10. Methods of genetic engineering 11. Sequenation of DNA, the significance in forensic practice 12. Transgenic organisms 13. Basics of genomics 14. Basic of bioinformatics

Conditions for subject completion

Full-time form (validity from: 2015/2016 Winter semester, validity until: 2019/2020 Summer semester)
Task nameType of taskMax. number of points
(act. for subtasks)
Min. number of pointsMax. počet pokusů
Graded credit Graded credit 100  51 3
Mandatory attendence participation:

Show history

Conditions for subject completion and attendance at the exercises within ISP:

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Occurrence in study plans

Academic yearProgrammeBranch/spec.Spec.ZaměřeníFormStudy language Tut. centreYearWSType of duty
2018/2019 (N2102) Mineral Raw Materials (3904T029) Mineral Biotechnology P Czech Ostrava 1 Compulsory study plan
2017/2018 (N2102) Mineral Raw Materials (3904T029) Mineral Biotechnology P Czech Ostrava 1 Compulsory study plan
2016/2017 (N2102) Mineral Raw Materials (3904T029) Mineral Biotechnology P Czech Ostrava 1 Compulsory study plan
2015/2016 (N2102) Mineral Raw Materials (3904T029) Mineral Biotechnology P Czech Ostrava 1 Compulsory study plan

Occurrence in special blocks

Block nameAcademic yearForm of studyStudy language YearWSType of blockBlock owner

Assessment of instruction

2017/2018 Winter
2016/2017 Winter
2015/2016 Winter