546-0841/01 – Instrumental Methods of Analysis II (IMA II)
| Gurantor department | Department of Environmental Engineering | Credits | 8 |
| Subject guarantor | doc. Mgr. Eva Pertile, Ph.D. | Subject version guarantor | doc. Mgr. Eva Pertile, Ph.D. |
| Study level | undergraduate or graduate | Requirement | Compulsory |
| Year | 3 | Semester | winter |
| | Study language | Czech |
| Year of introduction | 2015/2016 | Year of cancellation | 2015/2016 |
| Intended for the faculties | HGF | Intended for study types | Bachelor |
Subject aims expressed by acquired skills and competences
The objective of this course is to broaden knowledge gained in the course "Instrumental Methods I" and acquire further knowledge of instrumental analytical methods and enhance the preparedness of students for solving problems of selected instrumental analytical methods. The main aim of this course is to give an overview of selected analytical methods and procedures used in the analysis of real objects and chemometric evaluation of analytical results. Students can present and evaluate the analytical results, is aware of correct principles of working with an analytical sample, acquires training in practice in selected analytical calculations and experimental skills in analytical laboratory, basic knowledge about environmental sampling in connection with its share of the overall uncertainty by environmental analysis.
Teaching methods
Lectures
Experimental work in labs
Summary
The course is aimed at broadening and deepening knowledge of instrumental laboratory techniques, including acquiring practical skills. The main objective is to provide students with a solid and sufficiently broad theoretical and methodological basis for the method of instrumental analysis, qualitative and quantitative, both inorganic and organic compounds. The focus of teaching lies in the interpretation of the theoretical basics of instrumental methods of analysis. In practical exercises, the emphasis is on a individual approach of students to solve analytical problems of selected instrumental methods. Completing the course "Instrumental Methods II", along with completing the course "Instrumental Methods I" student acquires skills to work in the analytical laboratory with the basic instrumental equipment.
Compulsory literature:
Recommended literature:
Way of continuous check of knowledge in the course of semester
Knowledge control is checked in each exercise - check tests.
E-learning
Other requirements
Active participation in lectures and seminars.
Prerequisities
Subject has no prerequisities.
Co-requisities
Subject has no co-requisities.
Subject syllabus:
1. Introduction to instrumental methods, their advantages, disadvantages, the distribution of instrumental methods, their qualitative and quantitative use. Defining the problem. Basic terms. Processes of obtaining information. Absolute and comparative analytical methods. Calibration methods. Developments in instrumental methods of analysis.
2. Evaluating results of analysis and their expression. Statistical evaluation of analytical results. Reference material, a circular test. Fundamentals of chemometric approaches and methods. Testing the metrological characteristics of results.
3. - 5. Electroanalytical methods: their classification and principles, cells, electrode potential, Nernst and Peterson equation.
Methods based on measuring potential: Principles, instrumentation layout, uses, advantages and disadvantages. Electrodes and specific cells. Glass pH electrode, experimental approach to measure pH. Ion-selective electrodes. The choice of the electrode system. Nikolsky-Eisenman equation. Electrodes with additional membranes. Specific cell sets for potentiometric titration, evaluation of titration curves. Direct potentiometry, the method of calibration line, the method of standard additions, potentiometric titration, evaluation of titration curves, examples of use.
Methods based on current measurement: Voltammetry and polarography: Fundamentals of polarografic measurements: principle, instrument layout, qualitative and quantitative analysis, use. Mercury drop electrode and its properties. Currents, maximum. Sample treatment for polarographic measurements. Types of titration curves, their interpretation, applications. DC, AC, pulse, differential, inverse; curves shapes, evaluation, and charging the electrolytic current apparatus, calibration methods, examples of use. Current trends in voltammetry.
Amperometry, biamperometry - principles of methods, forms and evaluation of titration curves, examples of use. Electrogravimetry - electrolysis. Faraday's law, elimination of products on the cathode and anode, apparatus, examples of use. Coulometry: principles, coulometry at a constant potential, coulometry at a constant current, coulometric titration, evaluation, chronopotentiometry. Conductometry: mobility of ions, Kohlrausch law, specific and molar conductivity, direct conductometry, calibration, conductometric titration, shapes and evaluation of titration curves, examples of use. Principles, instrumentation, analytical applications, advantages and disadvantages.
6. - 9. Optical methods, interaction of matter and radiation, the classification of optical methods, principles, fundamentals of luminescence analysis, use of optical methods. Optical analytical methods: theoretical basics. Optical spectral apparatus. Non-spectroskopic methods - methods without enery changes between the substance and radiation, a) refractometry- refraction, method principle, apparatus, application examples, b) polarimetry - polarized light, optically active substance, the principle of the method, apparatus, examples of use.
Conditions for subject completion
Occurrence in study plans
Occurrence in special blocks
Assessment of instruction