546-0844/01 – Laboratory Practicum (LP)
| Gurantor department | Department of Environmental Engineering | Credits | 5 |
| Subject guarantor | doc. Mgr. Eva Pertile, Ph.D. | Subject version guarantor | doc. Mgr. Eva Pertile, Ph.D. |
| Study level | undergraduate or graduate | Requirement | Compulsory |
| Year | 1 | Semester | winter |
| | Study language | Czech |
| Year of introduction | 2016/2017 | Year of cancellation | 2020/2021 |
| Intended for the faculties | HGF | Intended for study types | Follow-up Master |
Subject aims expressed by acquired skills and competences
Knowledge rendered upon completion of the course: Obtaining increased knowledge and understanding of the reactivity of organic and inorganic compounds. Depending on the previous study it is to gain knowledge on sampling from different environments (eg.: surface water, soil, waste), and their further processing lab, including presentation and evaluation of the results.
Skills rendered upon completion of the course: After successfully completing the course will graduate a comprehensive overview and understanding of the theoretical and practical aspects in environmental fields.
Teaching methods
Experimental work in labs
Terrain work
Summary
The aim of the course is to broaden and deepen the knowledge of chemical laboratory techniques, including the acquisition of practical skills focused on the analysis of both waste, raw materials and other types of environmental samples (air, water, PM, green matter, etc.). The aim of the course is to deepen students' readiness to solve analytic problems by selected instrumental methods not only in the laboratory but also in the field. Provide an overview of selected analytical methods and procedures used to analyze real environmental samples and chemometric evaluation of analytical results.
Compulsory literature:
Recommended literature:
Additional study materials
Way of continuous check of knowledge in the course of semester
Successful processing of part-time assignments in the semester related to laboratory practice; computational and theoretical control tests. Laboratory tasks are chosen to always include individual types of laboratory techniques (sampling and sample preparation, spectrophotometry, volumetry, potentiometry, electrochemical separation methods, etc., including presentation and evaluation of obtained results). Each student works individually on the solved task and draws up a laboratory protocol, which he submits for inspection. The level and accuracy of the laboratory protocol is evaluated by a point assessment. Verification of the acquired knowledge is done by means of control tests (computational and theoretical) and the final credit test.
E-learning
Other requirements
Active participation in practice and submission of all protocols from laboratory tasks. Successful processing of partial tasks related to laboratory practice, which are given in the semester (sampling, sample preparation, etc.). Successful completion of written tests (computational and theoretical) prior to commencing a practical task when the student proves that he / she is sufficiently prepared to solve the given practical task. The requirement is also to successfully pass the final credit test.
Prerequisities
Subject has no prerequisities.
Co-requisities
Subject has no co-requisities.
Subject syllabus:
1. Introduction, work safety, laboratory glassware + utilities.
2. Basic laboratory work (weighing, precipitation, filtration, drying, titration, distillation, etc.); calculations: solution composition, titration factor, volumetric factor, pH value, basic statistical parameters.
3. Sampling and analysis of the waste sample: Sampling and sampling (preparatory part, sampling and laboratory sample preparation, sample handling, sampling documentation, liquid waste collection, viscous liquids collection, sludge collection, paste-like material picking, preparation sample mix, sample subtraction procedure, sample packaging and labeling, preservation of the sample). Preparation of aqueous extraction, determination of dry matter content and weight of analytical sample. Separation of liquid and solid phases, analysis of leachate and expression of results (according to methods for water-pH analysis, conductivity, ecotoxicity, DOC, phenolic index, ammonium ions, nitrates, nitrates, fluorides, chlorides, sulphates, Fe, Hg, Mn, Ni, Pb, Se, Zn).
4. Sampling and analysis of water samples: basic general rules, types of sampling, sample types, sampler, method of taking surface, underground, precipitation, wastewater and drinking water; sampling requirements, preservation of samples, transport and storage, pre-analysis. Drinking water analysis (basic concepts, microbiological and biological indicators, physical, chemical and organoleptic indicators). Surface water analysis (basic concepts, immission standards for indicators of permissible surface water pollution, ČSN 75 7221 classification of surface water quality). Basic water analyzes (taste, smell, color, temperature, transparency, turbidity, NK, COD, BOD, chlorine, ammoniacal nitrogen, nitrates, nitrates, Ca + Mg, Sulphates, Chlorides, phosphates, conductivity, pH etc.).
5. Soil sampling and analysis: Determination of basic concepts, factors and conditions of soil formation, soil composition, basic soil properties (color, structure, granularity, soil water, consistency, oxidation-reduction potential, nutrient content, humus content, soil sorption capacity, specific and bulk density, biological properties of soils). Sampling of soils (sampling procedure, adjustment of the volume of quaternary samples). Soil analysis (pH determination, H + fraction in sorption)
Soil complex by double pH measurement by Adam and Evans, determination of organically oxidizable carbon). Liquid analysis (determination of phosphates, Fe, Al, Ca, Mg, toxic metals - titration, colorimetry, AAS).
6. Titration methods: Jodometry (BOD, SAVO); Chelation (Ca + Mg); Neutralization titration (KNK, ZNK); Argentometry (Cl); Manganometry (COD); Conductometry (determination of acid and volumetric and conductivity).
7. Gravimetric determination: Sulphates, Fe, Ni, Mg, NL.
8. Distillation: Separation of organic substances, density of alcohol.
9. Solubility: Sample transfer into solution and relationships between structure and solubility.
10. Adsorption: Adsorption properties of activated carbon.
11. Spectrophotometry: Mn, PO4, Cr (VI), Turbidity, ammoniacal nitrogen.
12. Potentiometry: Determination of pH of samples, ISE.
13. Separation methods: TLC, GC, HPLC.
14. Field practice: Sampling, determination of physical, chemical and organoleptic indicators (Polanka) - use of mobile analytical methods.
Conditions for subject completion
Occurrence in study plans
Occurrence in special blocks
Assessment of instruction