617-2007/01 – Methods of Instrumental Analysis (MIA)

Gurantor departmentDepartment of ChemistryCredits6
Subject guarantorprof. Ing. Petr Praus, Ph.D.Subject version guarantorprof. Ing. Petr Praus, Ph.D.
Study levelundergraduate or graduateRequirementCompulsory
Year3Semesterwinter
Study languageCzech
Year of introduction2014/2015Year of cancellation
Intended for the facultiesFMTIntended for study typesFollow-up Master, Bachelor
Instruction secured by
LoginNameTuitorTeacher giving lectures
PAV57 Ing. Jiří Pavlovský, Ph.D.
PRA37 prof. Ing. Petr Praus, Ph.D.
VON37 Ing. Jiřina Vontorová, Ph.D.
Extent of instruction for forms of study
Form of studyWay of compl.Extent
Full-time Credit and Examination 2+3

Subject aims expressed by acquired skills and competences

The aim of the subject is to make students acquainted with basic methods of instrumental analysis. Students will be able to understand theoretical principals of instrumental methods and apply them in practice.

Teaching methods

Lectures
Experimental work in labs

Summary

The subject is focused on giving lessons of instrumental analytical methods, which are used in current laboratories. Teaching is conducted in a form of lectures and laboratory exercises.

Compulsory literature:

[1] G. D. Christian, Analytical Chemistry (5 th Ed.). New York , Wiley, 1994, 812 s. [2]D. Kealay, P.J: Haines, Analytical Chemistry. Oxford, BIOS Scientific Publishers, 2002, 342 s. [3]J. Kenkel, Analytical Chemistry for Technicians (4th Ed.). Boca Raton, CRC Press, 2014, 507 s.

Recommended literature:

1. Sommer L. a kol., Základy analytické chemie I. Skriptum, Brno, VUTIUM, 1998, 199 s. 2. Sommer L. a kol. Základy analytické chemie II. Skriptum, Brno, VUTIUM, 2000, 347 s. 3. Tomík B., Poljak B., Jirkovský R. Analytická chemie pro hutníky. Praha, SNTL, 1988, 264 s. 4. Vláčil F. a kol., Příklady z chemické a instrumentální analýzy Praha, SNTL, 1983, 432 s. 5. Churáček J. a kol., Analytická separace látek. Praha, SNTL, 1990, 384 s.

Way of continuous check of knowledge in the course of semester

E-learning

Další požadavky na studenta

Basic the knowledge of inorganic, organic and physical chemistry.

Prerequisities

Subject has no prerequisities.

Co-requisities

Subject has no co-requisities.

Subject syllabus:

Lectures 1. Properties of light, interaction of light with matter , refractive index (Cauchy equation)., light reflection and refraction, diffraction and interference of light refraction. Refractometry, interferometry and polarimetry. 2. Model of the atom, quantum numbers, Boltzmann law, the width of spectral lines. Dispersion of light, detection of electromagnetic radiation. 3. Atomic emission spectrometry, equipments, detectors, practical use. 4. Atomic absorption spectrometry, Lambert-Beer Law, methods of atomization, detectors, equipments, practical use. 5. Interaction of X-rays with matter, X-ray spectra, Auger effect, X-ray fluorescence spectrometry, sample preparation, X-ray dispersion, detectors, practical use. 6. X-ray diffraction, crystalline planes, Müller indexes, Debye-Scherrer method, evaluation of diffractograms, practical use. Electron Spectroscopy: Wehnelt cylinder, analyzers of electrons. Electron spectroscopy for chemical analysis (ESCA), Auger spectroscopy. 7. Interaction of molecules and electromagnetic irradiation, molecular spectra, LCAO theory, ligand field theory, charge -transfer complexes. UV-VIS absorption spectroscopy, luminescence spectroscopy, practical use. 8. Vibration of molecules, infrared spectrometry, equipments, FTIR spectroscopy, practical use. 9. Core in external magnetic field, NMR spectroscopy, equipments. Mass spectroscopy: ionization of molecules, fragmentation, separation of ions in the magnetic and electric field, the detection of ions, practical use. 10. Electrochemical potential (Peters and the Nernst equation), types of electrodes, direct potentiometry and potentiometric titration, practical use. Conductivity of strong and weak electrolytes, direct conductometry and conductometric titration, use in practice. 11. Electrolysis, Ilkovicova equation, polarography, AC-DC methods, voltammetry, practical use. 12. Separation methods in analytical chemistry, chromatography: principles of separation, van Deemterova and Giddings curve. Gas chromatography, liquid chromatography, practical use. 13. Migration of ions in electric field, ion electrophoretic mobility, electroosmotic flow. Plate and capillary electrophoresis, capillary isotachophoresis, isoelectric focusing, practical use. 14. Statistical treatment of analytical results, type of errors, normal distribution and its parameters, confidence intervals , statistical tests, reference materials. Laboratory exercises • Determination of resin exchange capacity • • Spectrophotometric study (determination of manganese and chromium in VIS region • Polarographic studies (determination of cadmium, nickel and zinc) • Potentiometric determination of chromium and vanadium in steel • Determination of cadmium and lead by atomic absorption spectrometry • Determination of sulphate by conductometric titration • Identification of simple organic compounds by gas chromatography • Determination of nitrate in waters by UV spectrophotometry • Refractometric determination of the Fridex concentration • Conductometric determination of specific electrolytic conductivity of water • Validation of methods for the determination of chromium in water - VIS spectrophotometry • Potentiometric determination of pH • Determination of nitrite in water - VIS spectrophotometry • Determination of chloride ion selective electrode • Determination of lead by amperometric titration • Identification of simple organic compounds by FT-IR spectroscopy (using the ATR technique) • Determination of elements in coal by X-ray fluorescence analysis

Conditions for subject completion

Full-time form (validity from: 2014/2015 Winter semester)
Task nameType of taskMax. number of points
(act. for subtasks)
Min. number of points
Exercises evaluation and Examination Credit and Examination 100 (100) 51
        Exercises evaluation Credit 45  21
        Examination Examination 55  6
Mandatory attendence parzicipation:

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Occurrence in study plans

Academic yearProgrammeField of studySpec.ZaměřeníFormStudy language Tut. centreYearWSType of duty
2020/2021 (N3923) Materials Engineering (3911T033) Material Recycling P Czech Ostrava 2 Compulsory study plan
2020/2021 (B3909) Process Engineering (3909R014) Process Engineering and Quality Control Methods P Czech Ostrava 3 Compulsory study plan
2019/2020 (N3923) Materials Engineering (3911T033) Material Recycling P Czech Ostrava 2 Compulsory study plan
2019/2020 (B3909) Process Engineering (3909R014) Process Engineering and Quality Control Methods P Czech Ostrava 3 Compulsory study plan
2018/2019 (N3923) Materials Engineering (3911T033) Material Recycling P Czech Ostrava 2 Compulsory study plan
2018/2019 (B3909) Process Engineering (3909R014) Process Engineering and Quality Control Methods P Czech Ostrava 3 Compulsory study plan
2017/2018 (B3909) Process Engineering (3909R014) Process Engineering and Quality Control Methods P Czech Ostrava 3 Compulsory study plan
2017/2018 (N3923) Materials Engineering (3911T033) Material Recycling P Czech Ostrava 2 Compulsory study plan
2016/2017 (N3923) Materials Engineering (3911T033) Material Recycling P Czech Ostrava 2 Compulsory study plan
2016/2017 (B3909) Process Engineering (3909R014) Process Engineering and Quality Control Methods P Czech Ostrava 3 Compulsory study plan
2015/2016 (B3909) Process Engineering (3909R014) Process Engineering and Quality Control Methods P Czech Ostrava 3 Compulsory study plan
2014/2015 (B3909) Process Engineering (3909R014) Process Engineering and Quality Control Methods P Czech Ostrava 3 Compulsory study plan

Occurrence in special blocks

Block nameAcademic yearForm of studyStudy language YearWSType of blockBlock owner