636-3027/02 – Special Microscopy Techniques (SMM)

Gurantor departmentDepartment of Material EngineeringCredits5
Subject guarantorprof. Ing. Vlastimil Vodárek, CSc.Subject version guarantorprof. Ing. Vlastimil Vodárek, CSc.
Study levelundergraduate or graduateRequirementChoice-compulsory type B
Study languageCzech
Year of introduction2019/2020Year of cancellation
Intended for the facultiesFMTIntended for study typesFollow-up Master
Instruction secured by
LoginNameTuitorTeacher giving lectures
VOD37 prof. Ing. Vlastimil Vodárek, CSc.
MAS0021 Ing. Anastasia Volodarskaja, Ph.D.
Extent of instruction for forms of study
Form of studyWay of compl.Extent
Full-time Credit and Examination 3+2

Subject aims expressed by acquired skills and competences

- explain principles of the most important microscopical techniques for characterization of engineering and biological materials, - explain possibilities and limitations of individual techniques, - learn, how to prepare specimens from engineering and biological materials, - demonstrate applications of modern microscopical techniques on practical examples.

Teaching methods

Experimental work in labs


The course is dealing with modern methods of structure characterization of materials which are exploited in materials and biomedical engineering. Students will learn about possibilities and limitations of individual experimental techniques, about methods of specimen preparation. Structure assessment of engineering and biological materials will be demonstrated on practical examples.

Compulsory literature:

WILLIAMS, D. B. and C. B. CARTER. Transmission electron microscopy, A textbook for materials science. 2nd edition, Springer US, 2012. ISBN 978-0-387-76502-0. DAWES, C. J. : Introduction to Biological Electron Microscopy: Theory and Techniques, Ladd Research Industries, Inc. Publisher Burlington, Vermont 1988.

Recommended literature:

GOLDSTEIN, J., et al. Scanning electron microscopy and X – ray microanalysis. 3rd edition, New York: Springer US, 2003. ISBN 978-0-306-47292-3.

Way of continuous check of knowledge in the course of semester

Průběžné ověření studijních výsledků: prezenční forma studia – 2 písemné testy, 2 programy zpracované v průběhu semestru; Závěrečné ověření studijních výsledků: prezenční forma - písemná zkouška.


Other requirements

Written report in the extent up to 20 pages on the given topic.


Subject has no prerequisities.


Subject has no co-requisities.

Subject syllabus:

1. Basic goals of structure characterization of materials in biomedical and materials engineering. 2. Products of electron interaction with a specimen. 3. X-ray absorption in solids. Diffraction of electrons on crystal lattice in cristalline materials. 4. Microscopy techniques based on focused electron beam. 5. Transmission electron microscope (TEM) – principle, spatial resolution, amplitude and phase contrast, diffraction analysis in TEM. 6. Problems related to investigations on biological specimens in TEM. Low voltage TEM. 7. Analytical electron microscopy. 8. Preparation of specimens for TEM from engineering materials(extraction replicas, thin foils – electrolytic thinning, FIB). 9. Preparation of specimens for TEM from biological materials (chemical and physical methods). 10. Scanning electron microscope (SEM): principle, spatial resolution and depth of focus, images in secondary and backscatter electrons, diffraction analysis in SEM. 11. Scanning electron microscopes with deteriorated vacuum level in the specimen chamber – low vacuum SEM (LVSEM) and environmental SEM(ESEM), possibilities and limitations of their applications for non-conductive specimens. 12. Preparation of biological specimens for SEM (crygenic and chemical methods). 13. X-ray microanalysis in SEM – energy and wave length dispersive analyses, qualitative and quantitative analyses, limits of detection. 14. Typical applications of electron microscopy in materials and biomedical engineering.

Conditions for subject completion

Full-time form (validity from: 2019/2020 Winter semester)
Task nameType of taskMax. number of points
(act. for subtasks)
Min. number of points
Credit and Examination Credit and Examination 100 (100) 51
        Credit Credit 30  15
        Examination Examination 70  36
Mandatory attendence parzicipation: Min. 80% attendance in exercises, preparation of the semesterial project.

Show history

Occurrence in study plans

Academic yearProgrammeField of studySpec.ZaměřeníFormStudy language Tut. centreYearWSType of duty
2020/2021 (N0788A270001) Biomechanical Engineering VSZ P Czech Ostrava 2 Choice-compulsory type B study plan
2019/2020 (N0788A270001) Biomechanical Engineering VSZ P Czech Ostrava 2 Choice-compulsory type B study plan

Occurrence in special blocks

Block nameAcademic yearForm of studyStudy language YearWSType of blockBlock owner