636-3027/02 – Special Microscopy Techniques (SMM)
| Gurantor department | Department of Material Engineering | Credits | 5 |
| Subject guarantor | prof. Ing. Vlastimil Vodárek, CSc. | Subject version guarantor | prof. Ing. Vlastimil Vodárek, CSc. |
| Study level | undergraduate or graduate | Requirement | Choice-compulsory type B |
| Year | 2 | Semester | winter |
| | Study language | Czech |
| Year of introduction | 2019/2020 | Year of cancellation | 2022/2023 |
| Intended for the faculties | FMT | Intended for study types | Follow-up Master |
Subject aims expressed by acquired skills and competences
- explain principles of the most important microscopical techniques for characterization of engineering and biological materials,
- explain possibilities and limitations of individual techniques,
- learn, how to prepare specimens from engineering and biological materials,
- demonstrate applications of modern microscopical techniques on practical examples.
Teaching methods
Lectures
Tutorials
Experimental work in labs
Summary
The course is dealing with modern methods of structure characterization of materials which are exploited in materials and biomedical engineering. Students will learn about possibilities and limitations of individual experimental techniques, about methods of specimen preparation. Structure assessment of engineering and biological materials will be demonstrated on practical examples.
Compulsory literature:
Recommended literature:
Way of continuous check of knowledge in the course of semester
Průběžné ověření studijních výsledků:
prezenční forma studia – 2 písemné testy, 2 programy zpracované v průběhu semestru;
Závěrečné ověření studijních výsledků:
prezenční forma - písemná zkouška.
E-learning
Other requirements
Written report in the extent up to 20 pages on the given topic.
Prerequisities
Subject has no prerequisities.
Co-requisities
Subject has no co-requisities.
Subject syllabus:
1. Basic goals of structure characterization of materials in biomedical and materials engineering.
2. Products of electron interaction with a specimen.
3. X-ray absorption in solids. Diffraction of electrons on crystal lattice in cristalline materials.
4. Microscopy techniques based on focused electron beam.
5. Transmission electron microscope (TEM) – principle, spatial resolution, amplitude and phase contrast, diffraction analysis in TEM.
6. Problems related to investigations on biological specimens in TEM. Low voltage TEM.
7. Analytical electron microscopy.
8. Preparation of specimens for TEM from engineering materials(extraction replicas, thin foils – electrolytic thinning, FIB).
9. Preparation of specimens for TEM from biological materials (chemical and physical methods).
10. Scanning electron microscope (SEM): principle, spatial resolution and depth of focus, images in secondary and backscatter electrons, diffraction analysis in SEM.
11. Scanning electron microscopes with deteriorated vacuum level in the specimen chamber – low vacuum SEM (LVSEM) and environmental SEM(ESEM), possibilities and limitations of their applications for non-conductive specimens.
12. Preparation of biological specimens for SEM (crygenic and chemical methods).
13. X-ray microanalysis in SEM – energy and wave length dispersive analyses, qualitative and quantitative analyses, limits of detection.
14. Typical applications of electron microscopy in materials and biomedical engineering.
Conditions for subject completion
Occurrence in study plans
Occurrence in special blocks
Assessment of instruction