651-2061/01 – Principles and Applications of Molecular Biotechnology (CAT/MBT)
| Gurantor department | Department of Chemistry and Physico-Chemical Processes | Credits | 3 |
| Subject guarantor | doc. RNDr. Jitka Frébortová, Ph.D. | Subject version guarantor | doc. RNDr. Jitka Frébortová, Ph.D. |
| Study level | undergraduate or graduate | Requirement | Compulsory |
| Year | 3 | Semester | winter |
| | Study language | Czech |
| Year of introduction | 2022/2023 | Year of cancellation | |
| Intended for the faculties | FMT | Intended for study types | Bachelor |
Subject aims expressed by acquired skills and competences
To introduce modern industrial, biomedical, pharmaceutical and agricultural applications of microbial and cell cultures. Competences acquired: student should be able to describe basic principles of molecular biotechnology, to describe principles of production of commercial products and therapeutics using genetically engineered microorganisms and cell cultures, and to describe the use of genetically engineered microorganisms for insect pest control, remediation and biomass utilization.
Teaching methods
Lectures
Other activities
Summary
Molecular biotechnology is a modern and dynamic field that exploits possibilities of manipulation with genetic information of the organism. The course describes experimental strategies leading to the expression of functional genes in prokaryotic and eukaryotic cells and to the production of authentic proteins useful as human therapeutics. The use of cell systems, especially microbial, as “biological factories” for the production of therapeutics, pharmaceutical proteins and nucleic acids, chemical compounds, antibiotics and biopolymers and the use of genetically modified microorganisms for biodegradation, biomass processing and agriculture are also demonstrated.
Compulsory literature:
Recommended literature:
Way of continuous check of knowledge in the course of semester
Oral exam.
E-learning
Other requirements
Requirements will be specified in lectures.
Prerequisities
Subject has no prerequisities.
Co-requisities
Subject has no co-requisities.
Subject syllabus:
1. Subject of molecular biotechnology. Microbial diversity and microorganisms used in biotechnology.
2. Molecular cloning.
3. Protein production in prokaryotic cells.
4. Protein production in eukaryotic cells. Part I: general characteristics of eukaryotic expression systems, yeast.
5. Protein production in eukaryotic cells. Part II: baculovirus, mammalian cells.
6. Protein engineering.
7. Large-scale production of proteins from recombinant microorganisms.
8. Molecular diagnostics.
9. Protein therapeutics. Nucleic acids as therapeutics.
10. Vaccines. Synthesis of enzymes, small biological molecules, antibiotics and biopolymers using recombinant microorganisms.
11. Bioremediation and biomass utilization.
12. Microbial insecticides.
Conditions for subject completion
Occurrence in study plans
Occurrence in special blocks
Assessment of instruction
Předmět neobsahuje žádné hodnocení.