651-2064/01 – Practice in material research methods (CAT/CMMV)
| Gurantor department | Department of Chemistry and Physico-Chemical Processes | Credits | 5 |
| Subject guarantor | Ing. Štěpán Kment, Ph.D. | Subject version guarantor | Ing. Štěpán Kment, Ph.D. |
| Study level | undergraduate or graduate | Requirement | Compulsory |
| Year | 3 | Semester | summer |
| | Study language | Czech |
| Year of introduction | 2022/2023 | Year of cancellation | |
| Intended for the faculties | FMT | Intended for study types | Bachelor |
Subject aims expressed by acquired skills and competences
The students will get acquainted with numerous techniques used for the purpose of the material and solid sample analysis.
Teaching methods
Experimental work in labs
Summary
The course aims to practice the knowledge of students acquired in the courses Physical-chemical methods of studying materials and some selected techniques discussed in the course Instrumental Analysis. This course is scheduled in the summer semester of the third year and is the last course focused on the characterization of materials, so the emphasis is on consolidating knowledge related to the discussed experimental techniques, including practical verification of their use in areas related to environmental technologies. Students will get acquainted with the following methods: determination of particle size distribution by selected techniques, methods of determination of specific surface area and pore size distribution, IR and Raman spectroscopy. The course will also demonstrate and practice techniques of XPS, AFM microscopy and Mössbauer spectroscopy, which, although not widely used in operating laboratories, have their undoubted importance in the field of material characterization and thus broaden the horizons of students in this study program. The task of determining the in vitro toxicity of nanomaterials using flow cytometry is also included among the laboratory exercises. Two exercises in the end are then focused on demonstrating a comprehensive approach to the analysis of water and waste materials, the last exercise is devoted to student presentations.
Compulsory literature:
Recommended literature:
Way of continuous check of knowledge in the course of semester
Credit.
E-learning
Other requirements
Requirements will be specified in laboratory exercises.
Prerequisities
Subject has no prerequisities.
Co-requisities
Subject has no co-requisities.
Subject syllabus:
Practical excercises:
1. Introductory lesson, work safety, acquaintance with practiced topics, acquaintance with laboratories.
2. Size characterization of powder materials using laser diffraction and dynamic light scattering method.
3. Use of transmission electron microscopy for size, morphological and structural characterization of materials.
4. Size and morphological characterization of nanoparticles using atomic force microscopy.
5. Determination of surface area and pore size distribution of nanocrystalline and porous materials.
6. IR spectroscopy in the characterization of some surface-modified nanoparticles - data processing, evaluation
7. Raman spectroscopy in the characterization of some surface-modified nanoparticles - data processing, evaluation.
8. Flow cytometry in the study of in vitro toxicity of nanomaterials. (introduction to nanotoxicology, study of the effect of nanomaterials on living systems, determination of cell viability by the Live / Dead method, determination of the effect of nanomaterials on the cell cycle).
9. Modern techniques of studying the chemical composition of sample surfaces - XPS, zeta potential, atomic force microscopy
10. Determination of chemical and physical parameters of samples in the liquid state (analysis of chemical composition, use of electrochemical methods in water analysis, etc.)
11. Analysis of industrial waste (treatment and preparation of samples for analysis, determination of basic parameters such as loss on drying and annealing, determination of chemical and phase composition,…).
12. Choice of procedure for characterization of unknown material, selection of methods and their principles, justification of the selection - presentation of students.
Conditions for subject completion
Occurrence in study plans
Occurrence in special blocks
Assessment of instruction
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