9360-0143/03 – Scanning probe microscopy and electron microscopy (SPM+EM)

Gurantor departmentCNT - Nanotechnology CentreCredits3
Subject guarantordoc. Ing. Vladimír Tomášek, CSc.Subject version guarantordoc. Ing. Vladimír Tomášek, CSc.
Study levelundergraduate or graduateRequirementCompulsory
Study languageEnglish
Year of introduction2013/2014Year of cancellation2019/2020
Intended for the facultiesUSPIntended for study typesFollow-up Master
Instruction secured by
LoginNameTuitorTeacher giving lectures
STE17 Ing. Gabriela Kratošová, Ph.D.
MAT27 doc. Ing. Vlastimil Matějka, Ph.D.
TOM24 doc. Ing. Vladimír Tomášek, CSc.
VAC121 Ing. Miroslav Vaculík, Ph.D.
Extent of instruction for forms of study
Form of studyWay of compl.Extent
Full-time Examination 2+0

Subject aims expressed by acquired skills and competences

The goal is to extend actual knowledge of students in electron microscopy and scanning probe microscopy and their applications in nanomaterials and nanotechnology sphere.

Teaching methods



The content of the subject is focused on the methods of materials structure study and their chemical and phase composition by transmission and scanning electron microscopy and scanning probe microscopy methods. The subject extend actual knowledge of students and parcicularly focuse on applications in nanomaterials and nanotechnology sphere. Students will be acquainted with the above mentioned imaging methods, with the principles of obtaining and processing of relevant image recordings and instrumentation of individual microscopes. Theoretically, they will acquaint themselves with instrumentation, aids and procedures for correct preparation of samples. They will also learn about combined techniques and correlative microscopy, it means techniques that combine different types of imaging with analytical methods. All information will be provided to students in relation to nanotechnologies and examples and specific images of nanoparticles / nanomaterials and applications in nanosciences will be documented.

Compulsory literature:

BONNELL, D. editor. Scanning Probe Microscopy and Spectroscopy, Theory, Techniques and Applications. Wiley-VCH, 2001. YAO, Nan, WANG, Zhong Lin. Handbook of Microscopy for Nanotechnology. Springer US, 2005. ISBN 978-1-4020-8003-6. WATT, I., M.: The Principles and Practice of Electron Microscopy. Cambridge University Press,1997.

Recommended literature:

WILLIAMS D. B. Practical Analytical Electron Microscopy in Materials Science. Verlag Chemie International, 1984. KRATOŠOVÁ, Gabriela, Kateřina DĚDKOVÁ, Ivo VÁVRA a Fedor ČIAMPOR. Investigation of nanoparticles in biological objects by electron microscopy techniques. In: Intracellular Delivery II: Fundamentals and Applications (Eds: Aleš Prokop, Y. Iwasaki, A. Harada), Springer Verlag, 2014.

Way of continuous check of knowledge in the course of semester


Other requirements

For this subject other requirements for student are not determined.


Subject has no prerequisities.


Subject has no co-requisities.

Subject syllabus:

- Interaction of electrons with matter - Basic types of electron microscopes - Function principle of transmition electron microscope - Sample preparation for transmition electron microscopy - Function principle of scanning electron microscope - Analytical electron microscopes - Interaction of ion beam with matter - Theoretical background in the field of scanning probe microscopy - Scanning tunneling microscopy - Atomic force microscopy - Other techniques of scanning probe microscopy

Conditions for subject completion

Full-time form (validity from: 2015/2016 Winter semester, validity until: 2019/2020 Summer semester)
Task nameType of taskMax. number of points
(act. for subtasks)
Min. number of points
Examination Examination 100  51
Mandatory attendence parzicipation: xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx

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Occurrence in study plans

Academic yearProgrammeField of studySpec.ZaměřeníFormStudy language Tut. centreYearWSType of duty
2019/2020 (N3942) Nanotechnology (3942T001) Nanotechnology P English Ostrava 1 Compulsory study plan
2018/2019 (N3942) Nanotechnology (3942T001) Nanotechnology P English Ostrava 1 Compulsory study plan
2017/2018 (N3942) Nanotechnology (3942T001) Nanotechnology P English Ostrava 1 Compulsory study plan
2015/2016 (N3942) Nanotechnology (3942T001) Nanotechnology P English Ostrava Compulsory study plan

Occurrence in special blocks

Block nameAcademic yearForm of studyStudy language YearWSType of blockBlock owner