9360-0208/01 – Molecular biology for nanotechnologists (MBN)
| Gurantor department | CNT - Nanotechnology Centre | Credits | 10 |
| Subject guarantor | Ing. Gabriela Kratošová, Ph.D. | Subject version guarantor | Ing. Gabriela Kratošová, Ph.D. |
| Study level | postgraduate | Requirement | Choice-compulsory |
| Year | | Semester | winter + summer |
| | Study language | Czech |
| Year of introduction | 2012/2013 | Year of cancellation | |
| Intended for the faculties | USP | Intended for study types | Doctoral |
Subject aims expressed by acquired skills and competences
The primary aim of this course is to introduce last trends in the field of molecular biology in their connection with nanotechnology, particularly with its application potential in nanobiotechnology and nanomedicine. Obtaining the general knowledge about biomolecules and their functions in living organism together with possible utilization in nanotechnology and nanomedicine will be emphasized. Students should be introduced to interdisciplinary fields and should recognize their importance and specifics – particularly in orientation in areas dealing with non-engineering and life-sciences such as molecular biology and biochemistry. This course in its some aspects is supplemental to „Biogenic materials and structures in nanotechnology“.
Teaching methods
Individual consultations
Summary
Nanotechnologies have become widely used in environmental engineering, medicine and the other fields dealing with life science. Molecular biology and genetic techniques can be utilized for study of mechanisms which are commonly used by living organisms. Cell performance and functions allow us
to understand the properties of nanosized matter and also the connection with the artificial nanotechnology.
Major molecular biology methods and approaches will be discussed with regard to experimental design and data analysis and with special interest for the cell behavior, different biomolecules as nanomotores (enzymes, proteins, amino acids, etc.) and applications in nanotechnology (bionanotechnology, nanobiotechnology, nanomedicine).
Compulsory literature:
1. D. S. Goodsell, Bionanotechnology, Lessons from Nature, John Wiley & Sons, Inc. 2004.
Recommended literature:
1. Challa S. S. R. Kumar Eds., Nanodevices for the Life Sciences, John Wiley & Sons, Inc. 2006.
2. C. M. Niemeyer and C. A. Mirkin Eds., Nanobiotechnology Concepts, Applications and Perspectives, John Wiley & Sons, Inc. 2005.
3. J. Cooper and T. Cass Eds., Biosensors - A Practical Approach, 2nd Ed., Oxford University Press 2004.
4. A. R. Freitas Jr., Nanomedicine, Basic Capabilities, Volume I, Landes Bioscience 1999, <http://www.nanomedicine.com/NMI.html>.
Way of continuous check of knowledge in the course of semester
E-learning
Other requirements
Write a seminar work. Topic of the seminar work is defined by supervisor.
Prerequisities
Subject has no prerequisities.
Co-requisities
Subject has no co-requisities.
Subject syllabus:
1. Characterization of the course, model organism in molecular biology, molecular level of genetics, characterisation, function, structures.
2. Nirenberg and Matthai experiment, molecular scissors, recombination technologies, PCR and application.
3. Genome sequencing, complete set of genetic information, different genomic approaches.
4. Gene therapy, pros, cons, cancer and tumour therapy, stem cell therapy.
5. Cell transport mechanism and permeability, cell membranes and functions, intracellular transport.
6. Cell signaling, cellular information processing, classification, realization, mechanism. Signaling pathways.
7. Cell cycle, cell cycle phases, cell differentiation process, cell ageing and death. Programmed cell death, apoptosis.
8. Biosensors. Biomolecules detection and signal recognition, surface immobilization of biomolecules, passivation.
9. Nanomedicine: hyperthermia, gene therapy, immunotoxins, drug delivery.
Conditions for subject completion
Occurrence in study plans
Occurrence in special blocks
Assessment of instruction
Předmět neobsahuje žádné hodnocení.