9360-0903/03 – Instrumental methods of analysis II (MIA)
| Gurantor department | CNT - Nanotechnology Centre | Credits | 10 |
| Subject guarantor | prof. Ing. Daniela Plachá, Ph.D. | Subject version guarantor | prof. Ing. Daniela Plachá, Ph.D. |
| Study level | postgraduate | Requirement | Choice-compulsory |
| Year | | Semester | winter + summer |
| | Study language | Czech |
| Year of introduction | 2019/2020 | Year of cancellation | |
| Intended for the faculties | FMT | Intended for study types | Doctoral |
Subject aims expressed by acquired skills and competences
Cílem předmětu je rozšíření znalostí v oblasti instrumentálních analytických metod zaměřených na kvalitativní a kvantitativní stanovení chemických látek, zejména v oblasti chromatografických a spektroskopických metod. Získané dovednosti lze následně aplikovat například při charakterizaci a testování účinnosti nově vyvíjených materiálů – sorbentů, katalyzátorů, fotokatalyzátorů, při sledování účinnosti nových technologických procesů a opatření vedoucích ke snížení znečištění životního prostředí.
Teaching methods
Lectures
Individual consultations
Summary
The lectures are aimed to increase the level of knowledge in the sphere of instrumental analytical methods of qualitative and quantitative determinations of organic compounds, especially of chromatographic and spectroscopic methods. The students will be able to apply the obtained knowledge for characterization and testing newly developped materials - sorbents, catalysts and photocatalysts, for observation of efficiencies of newly technological processes and methods for decreasing of environmental pollution.
Compulsory literature:
• McMASTER, M. C. GC/MS. Sec. Ed. Hoboken, USA: John Wiley & Sons, Inc., 2007.
• McMASTER, M. C. LC/MS, New York: John Wiley&Sons, 2005.
• McMAHON, G. Analytical Instrumentation, New York: John Wiley&Sons, 2007.
• SPARKMAN, O.D., Penton, Z.E., KITSON, F.G. Gas Chromatography and Mass Spectrometry,
Sed. Ed. Oxford: Elsevier, 2011.
• LUNDANES, E., REUBSAET, L., GREIBROKK, T. Chromatography: Basic Principles, Sample
Preparations and Related Methods, New York: John Wiley & Sons, 2013.
• ŠTULÍK, K., ŠEVČÍK, J., PACÁKOVÁ, V., JELÍNEK, I., COUFAL, P., BOSÁKOVÁ, Z.
Analytické separační metody, Praha: Nakladatelství Karolinum, 2005.
Recommended literature:
• McMASTER, M. C. GC/MS. Sec. Ed. Hoboken, USA: John Wiley & Sons, Inc., 2007.
• McMASTER, M. C. LC/MS, New York: John Wiley&Sons, 2005.
• McMAHON, G. Analytical Instrumentation, New York: John Wiley&Sons, 2007.
• SPARKMAN, O.D., Penton, Z.E., KITSON, F.G. Gas Chromatography and Mass Spectrometry,
Sed. Ed. Oxford: Elsevier, 2011.
• LUNDANES, E., REUBSAET, L., GREIBROKK, T. Chromatography: Basic Principles, Sample
Preparations and Related Methods, New York: John Wiley & Sons, 2013.
Additional study materials
Way of continuous check of knowledge in the course of semester
Study results will be verified by an oral exam.
E-learning
Other requirements
Student will be participate in chromatographic measurement.
Prerequisities
Subject has no prerequisities.
Co-requisities
Subject has no co-requisities.
Subject syllabus:
Theory of chromatografic processes.
Gas chromatography – instrumentation, stacionary phase, detectors, optimization of separation, sample derivatization vzorků, GCxGC analysis, applications.
Liquid chromatography – instrumentation, stacionary and mobile phase, interaction in liquid chromatography, detectors, optimization of separation, applications.
Supercritical fluid chromatography – theory of supercritical liquids, mobile and stacionary phasese, optimization of separation, applications.
Mass spectromery – theory, instrumentation, mass spectra, applications.
Gas chromatography in connection with mass spectrometry – ionization techniques, ion separation (kvadrupol detectors, ion trap, TOF), optimization of processes, applications. Methods of MS/MS.
Liquid chromatography in connection with mass spectrometry – ionization techniques, ion separation, optimization of mass detector parameters, applications. Methods of MS/MS.
Practical part:
Development and optimization of separation method, validation of methods, measurement uncertainety, evaluation of chromatographic patterns.
Conditions for subject completion
Occurrence in study plans
Occurrence in special blocks
Assessment of instruction